流动注射氢化物发生-原子吸收法测定乙肝疫苗中硫柳汞的含量

目的:建立测定乙肝疫苗中硫柳汞含量的方法。方法:采用流动注射氢化物发生-原子吸收法,以回流消化法处理样品,4%硫酸溶液为介质,0.5%硼氢化钾为还原剂,在0.5%硫酸作载液条件下,定量测定乙肝疫苗中的硫柳汞。结果:硫柳汞检测浓度的线性范围为5.0～100μg·L-1;平均回收率为100.6%,RSD=0.89%;最低检出限为1.00μg·L-1。结论:本方法简便、快速、灵敏度高,可用于乙肝疫苗中硫柳汞的含量测定。

Determination of Thiomersal in Hepatitis B Vaccine by Flow Injection-Hydride Generation Atomic Absorption Spectrometry

OBJECTIVE： To establish a method for the determination of the content of thiomersal in hepatitis B vaccine. METHODS： The content of thiomersal in hepatitis B vaccine was determined by flow injection hydride generation atomic absorption spectrometry. The samples were treated by reflux digestion method with 4% sulphuric acid as medium, 0.5% potassium borohydride as reducing agent, and 0.5% sulphuric acid as supporting liquid. RESULTS： The linear range of thiomersal was 5.0-100μg · L^-1, with an average recovery rate of 100.6% （RSD = 0.89% ） and the lowest detection limit of 1.00μg · L^-1. CONCLUSION： The method is simple, rapid and sensitive, and suitable for the determination of the content of thiomersal in hepatitis B vaccine.