葛根素对乳鼠心肌细胞缺氧-复氧损伤时NO产量增加的抑制作用

目的 :观察葛根素对缺氧 复氧损伤诱导的大鼠乳鼠心肌细胞一氧化氮 (NO)产生的作用。方法 :采用硝酸还原酶法测定对照组、模型组、及 1,0 .1,0 .01g·L^-1Pue给药组在各个时间点时细胞培养上清液中NO含量的变化。结果 :复氧开始后 ,模型组NO合成显著性地增加 (P<0 .0 1)。复氧后 6h ,NO合成激增 ,并一直保持。 3种剂量的Pue在复氧后 6h才表现显著性抑制 (P<0.01)NO合成的作用 ,并保持至其后 24h。结论 :在NO合成量激增时Pue才表现出显著降低缺氧 复氧损伤时心肌细胞分泌的NO ,并呈剂量依赖趋势。

Inhibitive Effect of Puerarin on Increased NO Production by Neonatal Cardiomyocytes During Hypoxia/Reoxygenation Injury

Objective: To investigate the effect of puerarin (Pue) on nitric oxide (NO) produced by neonatal rat cardiomyocytes during hypoxia/reoxygenation injury. Method: NO contents in the culture supernatants sampled from different groups (control, model, and therapeutic groups with 1 , 0.1 , 0.01 g·^-1 Pue) at different time were assayed with nitrate reductase method. Result: NO content of model increased after reperfusion (P<0.01), while it increased sharply at 6 h after reperfusion and kept thereafter. Only at 6 h after reperfusion did Pue in these three doses inhibite NO production (P<0.01) and kept to the 24 h after reperfusion. Conclusion: Pue exerts inhibitive effect only after NO production is enhanced sharply during hypoxia/reperfusion injury in a dose dependent trend.