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| 肝再生增强因子对肝星状细胞基因表达谱的影响 | |
| 引用本文： | 邵凤娟,成军,马英骥,郭江,张黎颖. 肝再生增强因子对肝星状细胞基因表达谱的影响[J]. 中国肝脏病杂志(电子版), 2013, 0(4): 42-46 |
| 作者姓名： | 邵凤娟 成军 马英骥 郭江 张黎颖 |
| 作者单位： | 哈尔滨医科大学附属第一医院感染科;首都医科大学附属北京地坛医院传染病研究所;新发突发传染病研究北京市重点实验室;哈尔滨医科大学附属第四医院感染科;首都医科大学附属北京地坛医院内五科; |
| 摘 要： | 目的将pcDNA3．1（-）．ALR（hALR）和pcDNA3．1（-）分别转染人肝星形细胞（HSC），筛选在人肝星形细胞中存在表达差异的基因，进一步研究hALR的生物学功能及机制。方法以脂质体技术将pcDNA3．1（-）．ALR和pcDNA3．1（-）分别转染人肝星形细胞系，提取总mRNA，逆转录为cDNA，应用基因表达谱芯片方法分析差异表达的基因。结果在所筛选的4096个基因中，有2个基因表达水平显著上调，25个基因表达水平显著下调。结论hALR对于人肝星形细胞基因表达谱有显著影响，为进一步研究hALR的生物学功能和作用机制提供了线索。 |
| 关 键 词： | 肝星形细胞 肝再生 基因表达谱 |
Effect of augmenter of liver regeneration on the gene expression profiling of hepatic stellate cells |
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| SHAO Feng-juan,CHENG Jun,MA Ying-ji,GUO Jiang,ZHANG Li-ying. Effect of augmenter of liver regeneration on the gene expression profiling of hepatic stellate cells[J]. Chinese Journal of Liver Diseases(Electronic Version), 2013, 0(4): 42-46 | |
| Authors: | SHAO Feng-juan CHENG Jun MA Ying-ji GUO Jiang ZHANG Li-ying |
| Affiliation: | 1.Department of Infectious Diseases, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; 2.Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beo＇ing 100011, China; 3.Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China; 4.Department of lnfectious Diseases, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China; 5.The Fifth Department of Internal Medcine, Beijing Ditan Hospital, Capital Medical University, Beijing 100011, China) |
| Abstract: | Objective To screening the differential gene expression in human hepatic stellate cells （HSC） treated with pcDNA3.1-ALR in order to investigate the biological functions and mechanism of human augmenter of liver regeneration （hALR）. Methods HSC were transfected by pcDNA3.1 （-） and pcDNA3.1- ALR, respectively, using FuGENE6 transfection reagent. The total mRNA was isolated and reverse transcribed to synthesize the cDNA probes. Analyzed the differentially expressed genes by microarray gene expression profiling technique. Results Two genes were up-regulated and 25 genes were down-regulated among detected 4096 genes. Conclusions hALR has marked effect on gene expression profilings of human HSC. These results provide new clues for studying the molecular biological function and mechanism of hALR. |
| Keywords: | Hepatic stellate cells Liver regeneration Gene expression profiling |
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