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| 以次黄嘌呤单核苷酸脱氢酶为靶点的新型抗结核药物高通量筛选模型的建立及应用 | |
| 引用本文： | 熊小椒,周爽,杨延辉,关艳,肖春玲. 以次黄嘌呤单核苷酸脱氢酶为靶点的新型抗结核药物高通量筛选模型的建立及应用[J]. 中国医药生物技术, 2011, 6(6): 11-449. DOI: 10.3969/cmba.j.issn.1673-713X.2011.06.011 |
| 作者姓名： | 熊小椒 周爽 杨延辉 关艳 肖春玲 |
| 作者单位： | 中国医学科学院医药生物技术研究所重点室, 北京,100050 |
| 基金项目： | 国家"重大传染病防治"科技重大专项 |
| 摘 要： | 目的 建立以结核分枝杆菌次黄嘌呤单核苷酸脱氢酶为靶点的新型抗结核药物高通量筛选模型.方法 以结核分枝杆菌H37Rv基因组为模板,pBEV表达质粒为载体,将guaB2基因克隆至pBEV以构建pBEV::guaB2重组表达质粒,表达并纯化重组的结核分枝杆菌次黄嘌呤单核苷酸脱氢酶;建立以测定反应体系340 nm吸光值变化速率... |
| 关 键 词： | 抗结核药 次黄嘌呤类 药物评价,临床前 |
Establishment and application of a novel high-throughput screening model targeting to inosine monophosphate dehydrogenase for antitubercular drugs |
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| XIONG Xiao-jiao,ZHOU Shuang,YANG Yan-hui,GUAN Yan,XIAO Chun-ling. Establishment and application of a novel high-throughput screening model targeting to inosine monophosphate dehydrogenase for antitubercular drugs[J]. Chinese Medicinal Biotechnology, 2011, 6(6): 11-449. DOI: 10.3969/cmba.j.issn.1673-713X.2011.06.011 | |
| Authors: | XIONG Xiao-jiao ZHOU Shuang YANG Yan-hui GUAN Yan XIAO Chun-ling |
| Affiliation: | XIONG Xiao-jiao,ZHOU Shuang,YANG Yan-hui,GUAN Yan,XIAO Chun-ling:State-Key Laboratory for Drug Screening,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050,China |
| Abstract: | Objective To establish a high-throughput(HTS) screening model targeting inosine monophosphate dehydrogenase(IMPDH) for the discovery of novel antitubercular drugs.Methods The H37Rv IMPDH coding gene guaB2 was amplified and cloned into pBEV expression vector.The recombinant GuaB2 protein was expressed in Escherichia coli BL21(DE3)pLysS and itsactivity was measured at 340 nm wavelength absorbance.HTS screening model was established based on the activity of GuaB2 and Z' factor was used to evaluate the quality ... |
| Keywords: | Anitubercular agents Hypoxanthines Drug evaluation preclinical |
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