雷公藤内脂醇联合热疗对Hep-2细胞的增殖抑制作用

目的：探讨雷公藤内脂醇(TL)联合热疗对导人喉癌细胞株（Hep-2）的增殖抑制率、细胞凋亡率、及细胞周期增殖各时相的影响，旨在为人喉癌治疗提供理论依据。方法：对数生长期Hep-2细胞随机分为对照组、单纯热疗组、单纯TL组及TL联合热疗组，应用MTT（噻唑蓝）摄入法检测Hep-2细胞增殖抑制率，流式细胞术检测细胞周期各时相细胞百分率变化及细胞凋亡率，电子显微镜观察亚细胞结构改变。结果：单纯热疗组、单纯TL组和TL联合热疗组Hep-2细胞增殖抑制率分别为21.2%、28.5%和54.5%，TL联合热疗组细胞增殖抑制率高于单纯热疗组和单纯TL组（P<0.01）。与对照组比较，单纯热疗组、单纯TL组和TL联合热疗组细胞周期进程发生明显改变，G1期细胞增多（P<0.01），S期细胞减少（P<0.01），而G2/M期细胞相对增多（P<0.01），细胞凋亡率升高（P<0.01）；与单纯热疗组、单纯TL组比较，TL联合热疗组上述各项指标变化差异均具有显著性（P<0.01）。电子显微镜观察，单纯热疗组、单纯TL组和TL联合热疗组Hep-2细胞核染色质趋边凝集，线粒体肿胀，可见凋亡小体改变；上述变化TL联合加热疗组轻于单纯热疗组、单纯TL组；对照组未见上述改变。结论：TL联合热疗可提高Hep-2细胞的增殖抑制率、抑制Hep-2细胞G1期向S期转化进程，诱导Hep-2细胞凋亡。

Growth inhibitory effect of triptolide combined with hyperthermia on Hep-2 cells

Objective To explore the effect of triptolide(TL) combined with hyperthermia on proliferation and apoptosis of Hep-2 cells and its affect on cell cycle phases,and provide theoretical foundation for treatment of laryngocarcinoma.Methods Hep-2 cells at logarithmic growth phase were randomly divided into control,hyperthermia,TL,TL combined with hyperthermia groups.MTT method was adopted to investigate the proliferation inhibition rate of the Hep-2 cells.Cell cycle and apoptotic rate of Hep-2 cells were analyze...