菌种筛选对1型肺炎链球菌产糖量的影响 |
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引用本文: | 黄放 邓杰 赵兆 王智杰 周宇 刘威 张珂 张伟 李小波 曾华英 江山 崔长法. 菌种筛选对1型肺炎链球菌产糖量的影响[J]. 国际生物制品学杂志, 2014, 37(3): 126. DOI: 10.3760/cma.j.issn.1673-4211.2014.03.006 |
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作者姓名: | 黄放 邓杰 赵兆 王智杰 周宇 刘威 张珂 张伟 李小波 曾华英 江山 崔长法 |
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作者单位: | 610023 成都生物制品研究所有限责任公司细菌性疫苗研究室(黄放、邓杰、赵兆、刘威、张伟、曾华英、江山、崔长法),科研开发部(王智杰、周宇、张珂),细菌性疫苗二室(李小波) |
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基金项目: | 国家国际科技合作项目(2012DF33450) |
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摘 要: | 目的 用无动物源性培养基筛选高产糖1型肺炎链球菌(Streptococcus pneumoniae serotype 1,PN1)。方法 配制无动物源性培养基,并用此培养基进行高产糖PN1单菌落筛选。比较筛选前后PN1在发酵培养中的产糖量。 结果 无动物源性培养基筛选前后PN1在发酵培养中的产糖量分别为420和960 mg/L,筛选后PN1的产糖量明显高于筛选前PN1。 结论 以无动物源性培养基筛选PN1可使PN1的产糖量明显提高。
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关 键 词: | 链球菌 肺炎 多糖类 细菌 培养基 菌种筛选 |
Effect of bacterial colony screening on polysaccharide yield of Streptococcus pneumoniae serotype 1 |
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Affiliation: | *Research Development of Bacterial Vaccine, Chengdu Institute of Biological Products Co., Ltd., Chengdu 610023, China |
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Abstract: | Objective To use the animal-free medium to screen a colony of Streptococcus pneumoniae serotype 1(PN1) with high polysaccharide yield. Methods The animal-free medium was prepared and used to screen a single colony of PN1 with high polysaccharide yield. The polysaccharide yield of PN1 in fermentation culture was compared between before and after screening. Results The polysaccharide yield of PN1 before and after screening with the animal-free medium was 420 and 960 mg/L in fermentation culture, respectively. The polysaccharide yield of PN1 was significantly higher significantly after screening than before screening. Conclusion PN1 screening with animal-free medium can significantly increase polysaccharide yield of PN1. |
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Keywords: | Streptococcus pneumoniae Polysaccharides bacteria Culture media bacterial colony screening |
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