MRL/lpr狼疮鼠骨TNF-α/NF-κB信号通路的表达

目的: 探讨系统性红斑狼疮小鼠(MRL/lpr)骨肿瘤坏死因子-α (tumor necrosis factor-α, TNF-α)/细胞核因子-κB p50 (nuclear factor-κB p50，NF-κB p50)信号通路的表达。方法: 选取C3He/HeJ小鼠(对照组)和MRL/lpr狼疮小鼠(狼疮组)各6只，饲养4个月后常规制备小鼠股骨组织切片，HE染色观察骨质情况，免疫组织化学染色检测TNF-α、NF-κB p50蛋白表达情况；密度梯度离心法分离和贴壁法筛选骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMMSCs)，然后培养；免疫细胞化学染色观察BMMSCs中TNF-α、NF-κB p50表达情况。结果： 与对照组比较，狼疮组小鼠骨小梁松散，出现骨质疏松，股骨TNF-α和NF-κB p50蛋白表达升高(P<0.05或P<0.01)，BMMSCs中TNF-α和NF-κB p50蛋白表达亦升高(P<0.05或P<0.01)。结论：狼疮鼠骨TNF-α/NF-κB信号通路处于异常活化状态。

Expression of TNF-α/NF-κB signaling pathway in the bone of systemic lupus erythematosus mice (MRL/lpr)

Objective: To investigate the tumor necrosis factor-α(TNF-α)/nuclear factor-κB p50 (NF-κB p50)signaling pathway in bone of systemic lupus erythematosus mice (MRL/lpr). Methods: Six C3He/HeJ mice (control group) and six MRL/lpr mice (lupus group) were feed, then mouse femur tissue sections were prepared and bone circumstances were observed by hematoxylin-eosin (HE) staining. The proteins of TNF-α and NF-κB p50 were detected by immunohistochemical staining. Bone marrow mesenchymal stem cells (BMMSCs) were isolated by density gradient centrifugation adherence screening method and cultured, then the expressions of TNF-α, NF-κB p50 were detected by immunocytochemistry staining. Results: Trabecular bone of MRL/lpr lupus mice showed osteoporosis and the percentage of the cortex to the volume of bone of MRL/lpr mice were significantly lower compared to control group (P<0.05). The protein expression levels of TNF-α and NF-κB p50 on the femur of MRL/lpr mice were higher than the C3He/HeJ mice (P<0.05 or P<0.01). Immunocytochemistry results displayed that on the BMMSCs of the MRL/lpr mice, the protein expression levels of TNF-α and NF-κB p50 were also higher than the C3He/HeJ mice (P<0.05 or P<0.01). Conclusion: TNF-α/NF-κB signaling pathway on the bone of lupus mice has been abnormally activated.