机械张力诱发小鼠增生性瘢痕的实验研究

目的观察机械张力诱发小鼠产生增生性瘢痕的可能性。方法小鼠背部形成2 cm长的手术切口,术后第4天,使用牵拉装置给予创面持续的张力刺激,维持2周后拆除装置,分别在牵引结束后1 d、30 d、60 d观察瘢痕大体形态及组织学形态变化。检测瘢痕面积、细胞数目及胶原量变化情况。结果牵拉后小鼠背部形成类似于人增生性瘢痕组织,并且瘢痕组织可以维持至少60 d,但瘢痕的面积有逐渐减少趋势。结论机械张力诱发的增生性瘢痕动物模型可以形成增生性瘢痕。

Experimental Study on Hypertrophic Scar Model Initiated by Mechanical Load

Objective To demonstrate whether scars initiated by mechanical load were visually and histopathologically identical to human hypertrophic scars. Methods A 2 cm linear full-thickness incision was made on the dorsum of the mouse. On postoperative day 4, tension on the wounds was created and lasted for 2 weeks. Macroscopic and histological characteristics were examined at day 1, 30, 60 after traction, respectively. The changes of scararea, velative collagen density and cellulartty were cal;culated. Results Resulting scars were structurally identical to human hypertrophic scars and were persisted for more than 60 days. However, area of scars was gradually shrinking. Conclusion Hyperthophic scar in animal model could be initiated by mechanical load.