应激状态下大鼠胃黏膜适应性细胞保护作用的研究

目的：探讨心理、一氧化氮合酶（NOS）及胃黏膜血流量（GMBF）与应激状态下大鼠胃黏膜适应性细胞保护相互作用及意义。方法：采用高压恒流脉冲刺激器复制胃黏膜损伤模型，将72只雄性SD大鼠随机分为对照组、规则光组及不规则光组3组，分光光度法检测NOS活性，动态检测GMBF及Nils Lambecht法判定胃黏膜损伤指数（Ⅱ）。结果：在两个应激组中，NOS均升高，GMBF均下降后回升，而黏膜损伤均渐趋减轻。在规则组中，Ⅱ与GMBF呈显著负相关，在不规则组中，Ⅱ与NOS和GMBF均呈显著负相关，而NOS与GMBF呈显著正相关。结论：NOS、GMBF及心理共同参与了胃黏膜适应性细胞保护反应。

Relation between nitric oxide synthase,psychology,gastric mucosal blood flow and gastric mucosal adaptive cytoprotection under stress in rats

Objective:To explore the relation between nitric oxide synthase(NOS),psychology,gastric mucosal blood flow(GMBF) and gastric mucosal adaptive cytoprotection under stress in rats and its possible mechanism.Methods:Animal model was constructed with impulsive stimulator of high voltage and stable currency, seventytwo male SD rats were randomly divided into three groups:control(C),regular(R) and irregular(I) group.NOS activity was measured by spectrophotometry and injure of gastric mucosa was measured by method of Nils Lambecht,GMBF was measured by LDF3 flowmeter. Results:In both R group and I group,NOS increased significantly,but injury index(II) was on contrary apparently decreasing.GMBF was increased gradually after stress.In R group,there was a negative proportion between II and GMBF,in I group,there was a negative proportion between II and GMBF and NOS,but there was a positive relationship between NOS and GMBF.Conclusion:NOS,psychology and GMBF all might participate in the course of the gastric mucosal adaptive cytoprotection under stress.