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| 锌对大鼠成骨细胞膜Ca2+-ATP酶活性的调节 | |
| 引用本文： | 岑小波,王瑞淑,王航.锌对大鼠成骨细胞膜Ca2+-ATP酶活性的调节[J].卫生研究,2000,29(1):52-54. |
| 作者姓名： | 岑小波 王瑞淑 王航 |
| 作者单位： | 1. 华西医科大学附属第一医院医学实验中心,成都,610041 2. 华西医科大学公共卫生学院 3. 华西医科大学口腔医学院 |
| 基金项目： | 国家自然科学基金!(No.39770647) |
| 摘 要： | 为了研究锌对大鼠成骨细胞膜Ｃａ＾２＋－ＡＴＰ酶和Ｎａ＾＋Ｋ＾＋－ＡＴＰ酶活尾及其机制，实验设计照组和３个补锌组。采用孔雀绿比色法同步测定膜Ｃａ＾２＋－ＡＴＰ酶和Ｎａ＾２＋，Ｋ＾＋－ＡＴＰ酶活性，以研究Ｚｎ＾２＋对酶活性的影响。 |
| 关 键 词： | 锌 Ca^2＋－ATP酶 成骨细胞 蛋白激酶C |
Zinc modulates the Ca2+-ATPase activity of rat osteoblast membrane |
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| Cen Xiaobo,Wang Ruishu,Wang Hang.Zinc modulates the Ca2+-ATPase activity of rat osteoblast membrane[J].Journal of Hygiene Research,2000,29(1):52-54. | |
| Authors: | Cen Xiaobo Wang Ruishu Wang Hang |
| Institution: | Medical Experimental Center of First University Hospital, West China University of Medical Sciences, Chengdu 610041, China. |
| Abstract: | The effect and mechanism of zinc on Ca 2 ATPase and Na ,K ATPase activity of rat osteoblast membrane were studied.The activities were measured by colorimetric method.The effect of special inhibitors of protein kinase C or calmodulin on membrane Ca 2 ATPase and Na ,K ATPase activity which induced by zinc were also examined.The results showed that zinc could increase membrane Ca 2 ATPase activity,but no influence was observed on Na ,K ATPase activity.R24571,a special inhibitor of calmodulin,had no effect on membrane Ca 2 ATPase activity. Calmodulin also could not promote the basic Ca 2 ATPase activity of membrane.But trifluoperazine,a special inhibitor of protein kinase C,obviously reduced its activity.It was concluded that the modulation of zinc on membrane Ca 2 ATPase activity of rat osteoblast could be mediated by protein kinase C. |
| Keywords: | zinc osteoblast Ca~(2 ) ATPase protein kinase C calmodulin |
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