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| 乌贼墨对H22癌细胞内Ca2+浓度、核膜Ca2+/Mg2+-ATP 酶活性及c-jun表达的影响 | |
| 引用本文： | 王成斌,孙克任,谢克勤,苏小游. 乌贼墨对H22癌细胞内Ca2+浓度、核膜Ca2+/Mg2+-ATP 酶活性及c-jun表达的影响[J]. 卫生研究, 2000, 29(2): 96-98 |
| 作者姓名： | 王成斌 孙克任 谢克勤 苏小游 |
| 作者单位： | 1. 中国预防医学科学院环境卫生监测所,北京,100021 2. 山东医科大学山东省毒理学重点实验室 |
| 基金项目： | 山东省卫生厅科学基金资助!( No.90 C0 83 0 ) |
| 摘 要： | 利用荧光探针和免疫组织化学方法，研究了乌贼墨对Ｈ２２癌细胞内ＣＡ＾２＋浓度，核膜Ｃａ＾２＋／Ｍｇ＾２＋－ＡＴＰ酶活性及ｃ－ｊｕｎ表达的影响。结果发现，乌贼墨使Ｈ２２癌细胞内Ｃａ＾２＋浓度分别降低了６９％和７９％，核膜Ｃａ＾２＋／Ｍｇ＾２＋－ＡＴＰ酶活性分别降低了２１％和３７％，ｃ－ｊｕｎ表达明显减少。 |
| 关 键 词： | 乌贼墨 肿瘤 抑制作用 H22癌细胞 钙 |
Effect of sepia on intracellular Ca2+ concentration, nuclear Ca2+/Mg2+-ATPase activities and c-jun expression in H22 cancer cells |
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| Wang Chengbin,Sun Keren,Xie Keqin,Su Xiaoyou. Effect of sepia on intracellular Ca2+ concentration, nuclear Ca2+/Mg2+-ATPase activities and c-jun expression in H22 cancer cells[J]. Journal of hygiene research, 2000, 29(2): 96-98 | |
| Authors: | Wang Chengbin Sun Keren Xie Keqin Su Xiaoyou |
| Affiliation: | Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China. |
| Abstract: | The effect of sepia on the intracellular Ca 2 concentration,nuclear Ca 2 /Mg 2 ATPase activities and the expression of c jun were studied in H 22 cancer cells by using fluorescent probe and immunohistochemical method.The results showed that intracellular Ca 2 concentration decreased 69% and 79%,nuclear Ca 2 /Mg 2 ATPase activities diminished 21% and 37%,c jun expression decreased obviously.The results suggested that sepia probably diminished the intracellular Ca 2 concentration,affected Ca 2 dependent nuclear Ca 2 /Mg 2 ATPase activities,thus reduced the amount of Ca 2 transporting into nuclei,so lessened the promoting effect of Ca 2 on c jun expression,and therefore inhibited cellular differentiation and proliferation.This might be one of the possible anti cancer mechanisms of sepia. |
| Keywords: | Ca 2 Ca 2 /Mg 2 ATPase c jun sepia |
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