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| 人支架蛋白IQGAP1-shRNA慢病毒载体的构建及鉴定 | |
| 引用本文： | 张芮,王一兵,林莉,王法刚,李强,曹永倩. 人支架蛋白IQGAP1-shRNA慢病毒载体的构建及鉴定[J]. 泰山医学院学报, 2013, 0(8): 561-564 |
| 作者姓名： | 张芮 王一兵 林莉 王法刚 李强 曹永倩 |
| 作者单位： | [1]山东医学高等专科学校病理教研室,山东济南250002 [2]山东大学附属省立医院烧伤整形美容外科,山东济南250021 |
| 基金项目： | 山东省自然科学基金（2006ZRB14130）;山东省优秀中青年科学家科研奖励基金（BS2011SW049）. |
| 摘 要： | 目的 制备携带人支架蛋白IQGAP1基因小分子干扰RNA的重组慢病毒.方法 分别设计合成针对IQGAP1的4个shRNA慢病毒干扰载体;分别转染293T细胞,利用Western blot验证shRNA的沉默效果,选择其中一个沉默效果较好的shRNA慢病毒干扰载体同源重组产生Lentivirus- IQGAP1-shRNA并测定病毒滴度.结果 测序证实,构建携带IQGAP1-shRNA的慢病毒载体具有较好的沉默靶基因的效果,包装慢病毒,病毒滴度为2.0×l010TU/ml.结论 成功制备携带IQGAP1-shRNA的重组慢病毒载体并包装出具高效感染力的慢病毒颗粒. |
| 关 键 词： | 支架蛋白1 RNA干扰 慢病毒载体 |
Construction and identification of RNA interference lentiviral vector for IQGAP1 gene |
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| ZHANG Rui,WANG Yi-bing,LIN Li,WANG Fa-gang,LI Qiang,CAO Yong-qian. Construction and identification of RNA interference lentiviral vector for IQGAP1 gene[J]. Journal of Taishan Medical College, 2013, 0(8): 561-564 | |
| Authors: | ZHANG Rui WANG Yi-bing LIN Li WANG Fa-gang LI Qiang CAO Yong-qian |
| Affiliation: | 1. Dept. of Pathology and Pathophysiology, Shandong Medical College, Jinan 250002, China; 2. Dept. of Bum and Plastic Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China) |
| Abstract: | Objective： To construct recombinant lentivirus with IQGAPl-short hairpin RNA （shRNA）. Methods： Four different kinds of lentivirns vectors containing IQGAPI-shRNA were designed and synthesized. 293T ceils were transfected by these IQGAPI-shRNAs, and the method of western blot was applied to verify the silence effects. One of four IQGAPI-shRNAs which had the best silence effect was chosen to recombine lentivirus vector and the titer of purified virus was deter- mined. Results： The titer of virus was 2.0×l010TU/ml. Conclusion： The lentivirus-IQGAPl-shRNA is constructed suc- cessfully. |
| Keywords: | IQGAP1 gene RNA interference lentivirus vector |
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