TRPV1通过非焦虑依赖的机制参与肠易激综合征内脏高敏感的形成

目的:探讨TRPV1受体是否通过影响焦虑水平,参与肠易激综合征(irritable bowel syndrome,IBS)内脏高敏感性的产生.方法:通过三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)单次灌肠的方法,分别使用野生型(wild type,WT)与Trpv1-/-小鼠构建...

TRPV1 contributes to visceral hypersensitivity in irritable bowel syndrome model induced by TNBS in a non-anxiety-dependent mechanism

Objective:To investigate whether TRPV1 receptor contributes to the visceral hypersensitivity in irritable bowel syndrome(IBS)through affecting the anxiety level.Methods:Wild-type(WT)and Trpv1^-/-mice were used to establish the IBS visceral hypersensitivity model by singular intrarectal administration of trinitrobenzene sulfonic acid(TNBS).Colorectal distension-abdominal withdrawal reflex(CRD-AWR)was used to detect the visceral sensitivity after the establishment of IBS model.Open field test and elevated plus maze were used to evaluate the anxiety level of mice.Results:The anxiety level of Trpv1^-/-mice was reduced compared with the WT mice both in the basal condition and in IBS model.However,compared with the basal condition,the anxiety levels of both Trpv1^-/-mice and WT mice were unchanged after the establishment of IBS model.Trpv1^-/-mice show reduced visceral hypersensitivity in IBS model compared with the WT mice.Conclusion:In singular intrarectal administration of TNBS induced IBS model,TRPV1 receptors may contributes to IBS visceral hypersensitivity through a non-anxiety-dependent mechanism.