芦荟素通过激活ERK1/2-Runx2信号通路介导对去卵巢大鼠骨量流失的保护作用

目的 观察芦荟素对去卵巢大鼠骨量的影响,并探讨可能的机制.方法 将30只雌性Sprague Dawley大鼠随机分为3组:假手术组(Sham)、去卵巢组(OVX)及去卵巢大鼠+芦荟素治疗组(LHS,去卵巢大鼠每天接受50 mg/kg芦荟素治疗,持续12周).12周后取双侧股骨进行微型计算机断层扫描(Micro-CT)检...

Aloin protects bone loss in ovariectomized rats by activating ERK1 / 2-Runx2 signaling pathway

Objective To observe the effect of aloin on the bone mass in ovariectomized rats and explore the possible mechanisms. Methods Thirty female Sprague Dawley rats were randomly divided into 3 groups: sham operation group (Sham); ovariectomized group (OVX) and ovariectomized rats + aloesin treatment group (LHS, ovariectomized rats received 50 mg / kg aloin for 12 weeks). After 12 weeks, the bilateral femurs were taken for micro-CT (Micro-CT), to detect the microscopic results of the femoral metaphysis, the changes in the results of trabecular bone were observed through histopathological sections, the changes in bone strength were detected through bone biomechanics, and the Western blot (WB ) was used to detect the possible mechanisms. Results The bone mineral density (BMD), bone microstructure, maximum load, and elastic modulus of the OVX group were significantly lower than those of the Sham group (P<0.05). After treatment with aloin, the bone density, bone microstructure, maximum load, and elastic modulus were significantly improved, which was statistically significant (P<0.05). WB detection showed that the expression levels of p-Erk1 / 2, Erk1 / 2, ALP, RUNX2, OCN and OPN in the OVX group were significantly lower than those in the Sham group (P<0.05). The expression levels of p-Erk1 / 2, Erk1 / 2, ALP, RUNX2, OCN and OPN in the LHS group were significantly higher than those in the OVX group. Conclusion Aloin can significantly improve the strength and bone mass of femurs in ovariectomized rats, and this effect may be achieved by activating the ERK1 / 2-Runx2 signaling pathway to promote osteogenesis.