原发性肾病综合征患者血清IL-2、IL-13的研究

目的　探讨原发性肾病综合征患者血清IL- 2、IL -13水平在肾病综合征发病中的作用及其临床意义。方法　采用ELISA法测定30例原发性肾病综合征患者口服强的松(1mg/kg) 8周治疗前后血清IL- 2、IL -13水平变化,并与正常对照组进行比较。同时对30例原发性肾病综合征患者进行肾活体组织检查。结果　30例肾病综合征患者治疗前血清IL 2水平(6 7.16±6 .5 5 )pg/ml,明显低于正常对照组(98.2 9±2 5 .96 )pg/ml(P <0 .0 5 ) ,血清IL 13水平(48.79±3.0 5 )pg/ml明显高于正常对照组(47.13±3.0 6 )pg/ml(P <0 .0 5 ) ;激素治疗后19例患者缓解(激素敏感型) ,11例患者无效(激素抵抗型)。激素敏感型患者治疗后血清IL- 2水平(72 .11±16 .95 )pg/ml与治疗前无显著差异,较正常对照组显著降低(P <0 .0 5 ) ,而治疗后血清IL 13水平(46 .37±3.32 )pg/ml较治疗前显著降低(P <0 .0 5 ) ,与正常对照组无显著差异;激素抵抗型患者治疗后血清IL- 2及IL-13水平均显著升高。结论　肾病综合征患者血清IL-2水平降低,血清IL- 13水平升高,两者均参与炎症过程,IL- 2、IL -13水平变化在一定程度上可提示病情变化,监测治疗效果。

Levels of IL-2 and IL-13 in serum of patients with nephrotic syndrome and their clinical significance

Objective To investigate the function and clinical significance of interleukin-2 (IL-2) and interleukin-13(IL-13) in patients with primary nephrotic syndrome (PNS).Methods Twenty healthy volunteers and thirty patients with PNS were included in this study.IL-2 and IL-13 levels of serum were evaluated by ELISA before treatment and after treatment with prednisone for 8 weeks.Simultaneously renal biopsy of thirty patients was obtained.Results Before treatment,IL-2 levels of serum were significantly lower than those of the control,IL-13 levels of serum were significantly higher.After treatment,IL-2 levels of serum were not significantly varied and IL-13 levels of serum were significantly lowered in nineteen cases who was sensitive to corticosteroids treatment.But IL-2 and IL-13 levels were elevated in eleven cases who were resistant to corticosteroids.Conclusion Serum levels of IL-2 in patients with PNS are lower than those in controls,IL-13 levels in patient with PNS are higher.They both participated the inflammatory reaction.The changes of IL-2 and IL-13 levels can reflect the condition of disease,and monitor the effect of therapy.