吡格列酮对胰岛素诱导血管平滑肌细胞增殖核抗原表达的影响

目的观察吡格列酮（Pio）对胰岛素（Ins）刺激的血管平滑肌细胞（VSMC）增殖的干预作用，及对增殖核抗原（PCNA）基因和蛋白表达水平的影响。方法（1）以四甲基偶氮唑盐比色法观察不同浓度、不同时间的Ins对VSMc增殖的影响，以及Pio和Ins共孵育对VSMC增殖的作用。（2）用半定量逆转录聚合酶链反应测定PCNAmRNA的表达。（3）用Westernblot法检测PCNA蛋白的表达。结果Ins促进VSMC增殖，促增殖效应在72h末达峰值，差异有统计学意义（P〈0．01）；而Pio能抑制Ins诱导的VSMC增殖及PCNAmRNA和蛋白表达水平，最佳浓度100μmol／L（P〈0．01）。结论 吡格列酮抑制胰岛素的促VSMC增殖作用，通过降低PCNA的表达影响VSMC表型转换，有望用于治疗动脉粥样硬化。

Effects of pioglitazone on insulin induced proliferating cell nuclear antigen(PCNA)expression of vascular smooth muscle cells

Objective To observe the interfering effect of pioglitazone on insulin-stimulated VSMCs proliferation as well as PCNA expression at both mRNA and protein levels. Methods 1. VSMCs were stimulated with insulin and/or pioglitazone at different concentrations for different periods of time, and the cell proliferation was determined by MTT. 2. The level of PCNA mRNA was measured by RT-PCR. 3. The protein level of PCNA was measured by Western blot. Results Insulin （10^-10 ～ 10^-7 mol/L） stimulated the proliferation of VSMCs, its peak effect appeared at 72h （P〈0.01 ）. While pioglitazone inhibited insulin-stimulated VSMCs proliferation and PCNA expression at both mRNA and protein levels, and its optimal effect appeared at 100μmol/L. Conclusions Pioglitazone can inhibit insulin-stimulated VSMCs proliferation and influence the phenotypic change of VSMCs by reducing PCNA expression, which has the therapeutic significance for treating atherosclerosis.