健脾益气中药对脾气虚证2型糖尿病大鼠胰岛素介体的影响

目的观察健脾益气中药对脾气虚证2型糖尿病大鼠肝细胞膜胰岛素介体的影响.方法采用中剂量链脲佐菌素(30mg/kg,qd×3)腹腔内注射与过度劳倦、饥饱失常及肥甘饮食的方法造成2型糖尿病病证结合(脾气虚证)大鼠模型,设中药治疗组、模型组及正常对照组,治疗前及治疗3周后检测空腹血糖、血浆胰岛素,并于治疗结束后检测肝细胞膜胰岛素介体释放量.结果中药治疗组与模型组比较,肝细胞膜介体释放量显著增加(P<0.05),中药治疗前后比较,空腹血糖明显下降(P<0.05),胰岛素敏感性显著上升(P<0.05).结论健脾益气中药能增加肝细胞膜释放的抑制腺苷酸环化酶活力的胰岛素介体量,改善高胰岛素血症,提高胰岛素敏感性.

Effect of Spleen- strengthening and Qi- Invigorating Herbal Medicines on Insulin Mediator in Type 2 Diabetic Rats with Spleen- Qi Deficiency Syndrome

Objective To observe the effect of the spleen-strengthening and qi-invigorating herbal medicines on insulin mediator in type 2 diabetic rats with spleen-qi deficiency syndrome.Methods Rat models were induced by intraperitioneal injection of streptozocin (STZ),overstrain,abnormal meals with greasy foods.Model rats were randomly allocated to two groups:Group A was treated with herbal medicine with the actions of strengthening spleen and invigorating qi;Group B was treated with purified water.The normal rats were allocated to the control group.The serum contents of fasting blood-glucose and insulin were observed before and after 3-week treatment.The content of hepatic cell-membrane insulin mediator was observed after 3-week treatment.Results After 3 weeks of treatement the serum contents of fasting blood-glucose were decreased and the sensitivity of serum insulin were increased in Group A as compared with those before treatment (P < 0.05).The content of hepatic cell-membrane insulin mediator was increased in Group A as compared with Group B(P < 0.05).Conclusion The herbal medicine with the actions of strengthening spleen and invigorating qi is effective in increasing the content of the hepatic cell-membrane insulin mediator and improving the sensitivity of serum insulin in diabetic rat models with the spleen-qi deficiency syndrome.