| 尿液中羟基红花黄色素A的含量测定 |
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| 引用本文： | 曾志平,王实强.尿液中羟基红花黄色素A的含量测定[J].海南医学院学报,2010,16(1):10-12. |
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| 作者姓名： | 曾志平 王实强 |
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| 作者单位： | 1. 广州军区总医院,广东,广州,510010
2. 湖南省中医药研究院中药研究所,湖南,长沙,410013 |
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| 摘 要： | 目的:建立高效液相色谱法用于测定人尿液中羟基红花黄色素A的含量。方法:采用乙腈∶0.2%磷酸水溶液(20∶80 v/v)为流动相,流速为1 mL/min,检测波长为400 nm。结果:羟基红花黄色素A的线性范围为0.58～57.69μg/mL,羟基红花黄色素A在尿中3个浓度的绝对回收率分别平均为100.3%、100.8%、100.9%。结论:本方法简单快速,准确灵敏,重现性好,适用于羟基红花黄色素A的尿中药物浓度测定。
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| 关 键 词： | HPLC法 羟基红花黄色素A 含量 高效液湘色谱法 |
Determination of HYSA in human urine by phase high-performance liquid chrornatography |
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| ZENG Zhi-ping,WANG Shi-qiang.Determination of HYSA in human urine by phase high-performance liquid chrornatography[J].Journal of Hainan Medical College,2010,16(1):10-12. |
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| Authors: | ZENG Zhi-ping WANG Shi-qiang |
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| Institution: | 1. Guangzhou General Hospital of Guangzhou Military Corn mand , Guangzhou 510010, China; 2. Research Institute of Chinese Herb, Hunan Academy of Chinese Traditional Medicine, Changsha 410013, China) |
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| Abstract: | Objective; To establish a phase high-performance liquid chromatography for determina- tion of hydroxysafflor yellow A （HYSA） level in human urine. Methods： A ratio of acetonitrile to 2% phosphoric acid solution （10 ： 90, V/V） was used as the mobile phase at a flow rate of 1.0 mL/min with detection wave length being 400 nm. Results： It showed that HYSA had a good linear relationship when its concentration was between 0.58-57.69 μg/mL, and the absolute recoveries were 100.3%, 100.8% and 100.9% respectively. Concolusion： The method was simple, quick and convenient for determination of HYSA in human urine. |
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| Keywords: | HPLC method Hydroxysafflor yellow A （HYSA） Concentration High-performance liquid chromatography |
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