HPLC法测定人血浆中头孢吡肟的浓度

目的:建立以高效液相色谱法测定人血浆中头孢吡肟浓度的方法。方法:色谱柱为DiamonsilC18,流动相为20mmol.L-1醋酸铵溶液(冰醋酸调节pH值至5.00)-乙腈(90:10),流速为1.0mL.min-1,检测波长为254nm,进样量为20μL,柱温为室温。结果:头孢吡肟血药浓度在0.2～50.0μg.mL-1范围内线性关系良好(r=0.9996),定量下限为0.2μg.mL-1;平均方法回收率为99.16%(RSD=1.29%),平均提取回收率为85.43%(RSD=2.37%),日内、日间RSD均<10%。结论:本方法简便、灵敏、快速、准确,可用于人血浆中头孢吡肟的药动学和生物利用度研究。

Determination of Cefepime in Human Plasma by HPLC

OBJECTIVE:To establish an HPLC method for the determination of cefepime in human plasma.METHODS:The separation of sample was carried on a Diamonsil C18 column with the mobile phase consisted of 20 mmol·L-1 ammonium acetate(which was adjusted to pH=5.00 by acetic acid)-acetonitrile(90∶10) at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm;the sample size was 20 μL,and the column temperature was set at room temperature.RESULTS:The linear range of cefepime was 0.2～50.0 μg·mL-1(r=0.999 6),with the lower limit of quantitation at 0.2 μg·mL-1.The average methodological recovery was 99.16%(RSD=1.29%) and the average extraction recovery was 85.43%(RSD=2.37%).The inter-day RSD and intra-day RSD were all less than 10%.CONCLUSION:The method was simple,sensitive,rapid and accurate and can be used for the pharmacokinetic and bioavailability study of Cefepime.