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Repair of bone defects around dental implants with bone morphogenetic protein/fibroblast growth factor‐loaded porous calcium phosphate cement: a pilot study in a canine model |
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| Authors: | Shijie Zhang Jun Zhao Kefeng Pan Yuanliang Huang |
| Affiliation: | 1. Department of Stomatology, the Second Hospital Affiliated to Shandong University, Jinan, Shandong, China;2. Department of Oral and Maxillofacial Surgery, School of Medicine, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China;3. Department of Oral and Maxillofacial Surgery, School of Stomatology, Tongji University, Shanghai, China;4. Department of Stomatology, East Hospital Affiliated to Tongji University, Shanghai, China |
| Abstract: | Objectives: To study the effects of the bone morphogenetic protein‐2 (BMP‐2)/fibroblast growth factor (bFGF)‐loaded porous calcium phosphate cement (CPC) on the repair of bone defects around dental implants. Material and methods: Thirty critical‐sized bone defects in beagle dogs were repaired with engineered bone composed of autologous bone‐marrow‐derived mesenchymal stem cells (BMSCs), BMP‐2, bFGF and CPC. Repairs were divided into six groups: BMSC/BMP‐2/bFGF/CPC, BMSC/BMP‐2/CPC, BMSC/bFGF/CPC, BMSC/CPC, CPC and no treatment. Polychrome sequential fluorescent labels were also performed post‐operatively. Fluorescence histological examinations of undecalcified sections at post‐operative week 12 were performed to clarify changes in the new bone around the dental implants. Results: The animals exhibited a perfect post‐operative course, with none experiencing any infection. Undecalcified sections showed that new bone was actively formed in the BMP‐2/bFGF group after 12 weeks. The bone mineralization apposition rate was better in the BMP‐2/bFGF group than in the other groups (P<0.05). Conclusion: BMP‐2 and bFGF together are more effective than either one alone in promoting the formation of new bone and may exert a synergistic activity at bone defects around dental implants. To cite this article: Wang L, Zou D, Zhang S, Zhao J, K Pan, Huang Y. Repair of bone defects around dental implants with bone morphogenetic protein/fibroblast growth factor‐loaded porous calcium phosphate cement: a pilot study in a canine model. Clin. Oral Impl. Res. 22 , 2011; 173–181. doi: 10.1111/j.1600‐0501.2010.01976.x |
| Keywords: | basic fibroblast growth factor bone defect bone morphogenetic proteins dental implants porous calcium phosphate cement tissue engineering |