晶体蛋白质的混浊与PH值的关系

我们使用一些酸性试剂及药物,如HCl:谷胱甘肽(GSH,GSSG);维生素C;维生素B_6等,与碱性试剂(Tris)来调节晶体水溶性和尿素溶性蛋白质的pH值,发现在一定比例下,晶体蛋白质溶液产生混浊,用pH计测混浊溶液的pH值约为5.6—6.3,高于或低于这一范围,混浊不会产生.通过酸、碱梯度溶液试验,测定了人类胚胎晶体、成人透明和白内障晶体水溶性和尿素溶性蛋白质溶液的混浊程度,其结果是,成人透明晶体和白内障晶体蛋白溶液比胚胎晶体蛋白溶液产生的混浊明显,在同一晶体中,尿素溶性蛋白比水溶性蛋白产生的混浊多.我们认为白内障的形成与晶体内pH变化有一定联系.

RELATIONS BETWEEN OPACIFICATION OF LENS PROTEIN AND pH

Some of the acidic reagents or medicines (i. e. HCl,GSH & GSSG, Vit. C,Vit. B_5) and basic reagents (Tris) were used to regulate the pH of water soluble protein and urea soluble protein solution of human lenses. At a centain pH,the lens protein solution appears opalescence, the pH of opacity solution which is measured by pH meter is around 5.6-6. 3. The opalescence of water soluble and urea soluble lens protein solution of human fetal, adult and cataractous lenses were also determined by gradient acidic and basic solution. The result show that the opacity is more obvious in soluble protein solution of cataractous lenses and clear lenses than in that of fetal lenses,and the lens urea soluble protein has a marked opalescence in comparison with the water soluble protein. So we assume that cataractogenesis might be associated with the change of pH within lens.