清胰汤对大鼠重症急性胰腺炎炎症反应的影响

目的研究清胰汤对大鼠重症急性胰腺炎（SAP）炎症反应的影响。方法将45只健康雄性sD大鼠随机分为假手术组、模型组及清胰汤组，每组15只。假手术组：常规饲养，经胰管逆行注射生理盐水，不给药。模型组：用5％牛磺胆酸钠经胰管逆行注射（1mg／kg）构造SAP模型，喂饲无菌蒸馏水，4ml／次，每12小时一次。清胰汤组：复制SAP模型，喂饲等量清胰汤。建模24h后每组处死7只大鼠，胰腺组织切片，观察其病理学改变。同时检测其余大鼠血淀粉酶、血钙以及血清肿瘤坏死因子α（TNF-α）、白细胞介素8（IL-8）的浓度。结果清胰汤组TNF-α、IL-8水平明显低于模型组（P均〈0．05），但高于假手术组（P均〈0．05）。清胰汤组胰腺组织的病理损害明显轻于模型组。结论对SAP大鼠给予清胰汤能抑制其释放TNF-α、IL-8，进而起到一定的治疗作用。

Therapeutic effect of Qingyi decoction on changes of inflammatory factor in rat serum with severe acute pancreatitis

Objective To investigate the effect of Qingyi decoction on inflammation reaction in rats with severe acute pancreatitis （SAP）. Methods Fourty-five male SD rats were randomly divided into the sham-operated group, model group, and Qingyi decoction group, 15 rats in each group. The sham-operated group received normal saline infusion into biliopancreatic duct. SAP was induced in the model group and Qingyi decoction group by retrograde injection of 5% sodium taurocholate （1 mg / kg body weight） into biliopancreatic duct. The rats in Qingyi decoction group were orally administered Qingyi decoction while those in the model group given sterile distilled water instead, both at a dose of 4 ml every 12 hours. Seven rats in each group were sacrificed 24 hours after SAP modeling, and the histopathological changes in pancreatic tissues were observed. At the same point time, the levels of serum amylase, Ca^2＋, tumor necrosis factor-α（TNF-α）, interleukin-8 （IL-8） of the other 24 rats were measured. Results The levels of serum amylase, TNF-α and IL-8 were remarkably lower in Qingyi decoction group comparing with those in the model group （all P〈 0.05）, while they were much higher than those in the sham-operated group （all P 〈 0.05）. The level of serum Ca^2＋ was obviously higher in Qingyi decoction group than the model group. When compared with the model group, the pathological manifestations in the pancreatic tissues were significantly attenuated in the Qingyi decoction group. Conclusion It indicated that Qingyi decoction can inhibit the release of TNF-α and IL-8 to reduce inflammation of SD rats with SAP.