磷酸羟基哌喹对血液系统辐射损伤保护作用的实验研究

目的研究磷酸羟基哌喹对辐射损伤小鼠血液系统的保护作用。方法40只昆明小鼠,随机分为5组（HPQP低、中、高剂量组,空白对照组和正常组）,除正常组外,其余各组给予4.5 Gy60Co-γ射线全身照射,以外周血白细胞、血小板计数观察辐射后小鼠的血液系统变化,初步观察药物对血液系统的影响,并筛选出药物的合适剂量;另取24只小鼠,随机分为3组（用药组、对照组、正常组）,以此前确定的磷酸羟基哌喹最适剂量于照射前灌胃给药,予9.0 Gy60Co-γ射线全身照射,照射后第9天处死小鼠,以脾结节法、骨髓有核细胞计数法、骨髓病理观察药物对小鼠造血功能的影响。结果磷酸羟基哌喹可促进辐射后小鼠白细胞恢复（P〈0.01）,降低死亡率（P〈0.01）,使脾结节（CFU-S）数、骨髓有核细胞数明显增加（P〈0.01）。结论磷酸羟基哌喹对辐射损伤小鼠血液系统有保护作用。

Experimental study of the protective effect of hydroxypiperquin phosphate on hematological system injury induced by radiation exposure in mice

Objective To investigate the protection effect of hydroxypiperquin phosphate（HPQP） on hematological system injury induced by radiation damage in mice.Methods 40 male Kunming mice were randomly divided into five groups（low/middle/high dose groups,blank control group and normal group）.All except the normal group underwent whole-body γ-ray irradiation at 4.5 Gy.The peripheral leukocyte and platelet counts were determined for the primary effect on hematological system to screen out the most suitable dose of the drug.Meanwhile,another 24 mice were randomized into 3 groups（drug treatment group,control group and normal group）,which underwent gastrogavage at the previously determined optimal dose of HPQP prior to irradiation at 9.0 Gy γ-ray except the normal group.All the mice were sacrificed 9 days after radiation,and the effects of the drug on the hematopoietic function were evaluated by spleen colony method,bone marrow nucleated cell counts and observation of the bone marrow pathology.Results Hydroxypiperquin phosphate can promote the post-radiative restoration of peripheral leukocytes to a normal level（P0.01） in mice,decreased mortality（P0.01）,and markedly increased colony-forming unit-spleen（CFU-S） and bone marrow nucleated cell counts（P0.01）.Conclusion Hydroxypiperquin phosphate has protective effect on the radiation-induced damage in mice;mice