| Abstract: | Organ tissue cultures of human mammary carcinomas were performed for twenty-four, forty-eight, and seventy-two hours. All types of tumor tested produced growth that could be quantitated by labeling with tritiated thymidine. Incorporation was located mainly within epithelial cells as controlled by autoradiography. Two labeling methods were compared, Parker 199 and Trowell's T8 media were tested, and the addition of human albumin or serum was evaluated. The serum factor was important for growth, although no difference between Parker 199 and Trowell's T8 medium could be demonstrated. The data were suitable for statistical evaluation, and variance analysis showed the tumors, culture times, and media to be significant sources of variation. The method is intended for studies of hormonal responsiveness of human mammary carcinomas. |
