EGCG抑制口腔鳞癌Tca8113细胞增殖和诱导凋亡的研究

目的：研究绿茶提取物表没食子儿茶素没食子酸酯（EGCG）对人口腔鳞癌Tca8113细胞生长抑制及促凋亡的作用。方法：应用MTT法检测不同浓度的EGCG对Tca8113细胞体外增值抑制作用;采用HE染色、Hochest33258染色法检测EGCG对Tca8113细胞凋亡的影响。结果：MTT法检测显示EGCG对Tca8113细胞有明显增殖抑制作用并呈现时间及浓度依赖性（P〈0.05）;HE、Hochest33258染色显示,EGCG处理Tca8113细胞中出现了典型的凋亡形态学改变如核碎裂、核浓缩。结论：EGCG对Tca8113细胞的生长具有显著的抑制及促凋亡作用,且呈现时间及浓度的依赖性。

EFFECT OF EGCG ON THE GROWTH INHIBITION AND APOPTOSIS OF HUMAN ORAL SQUAMOUS CELL CARCIONMA TCA8113

Objective：Table of green tea extract epigallocatechin gallate （EGCG） Tca8113 human oral squamous cell growth inhibi- tion and pro - apoptotic effects. Methods： MTI~ assay was used to detect different concentrations of EGCG value - added TcaSll3 cells in vitro inhibition ; HE staining, Hochest33258staining EGCG Tca8113 apoptosis. Results： The MTr assay Display EGCG significantly inhibited the proliferation and render time and concentration dependence on Tca8113 cells（ P 〈 0.01 ） ; HE, Hochest33258 staining re- vealed, EGCG treatment TcaSll3 cells, typical apoptotic morphological changes such as nuclear fragmentation, nuclear enrichment. Condusion：EGCG has obvious proliferation inhibition and apoptosis of rendering time and concentration dependence of TcaS113 cells.