环孢霉素A对脑缺血再灌注损伤的保护作用

目的：探讨环孢霉素A（Cyclosporin,CsA）对局灶性脑缺血再灌注损伤大鼠的神经保护作用。方法：雄性SD（Spregue-Dawley）大鼠随机分组,测定脑组织含水量的大鼠分为治疗组、损伤对照组和正常对照组。测定脑梗死灶面积的大鼠分为治疗组和损伤对照组。采用改良的Longa法制作脑缺血再灌注动物模型。术后1h予以再通。2治疗组大鼠均于损伤前5d（包括损伤当天）臀股内按15mg(kg.d）注入CsA生理盐水溶液,2损伤对照组大鼠均注和相同剂量的生理盐水,正常对照组未损伤,未用药。术后24h行TTC染色测定大鼠脑梗死区面积。术后48h测定梗死区脑组织水量。结果：CsA治疗组和对照组相比,梗死灶面积缩小（P＜0．01）,脑水肿减轻（P＜0．01）。结论：CsA对脑缺血再灌注损伤大鼠有明显的神经保护作用。

Protective effects of cycolsporin A on cerebral ischemia reperfusion injury in rats

Aim: To investigate the neuroprotective effects of cycolsporin A after focal cerebral ischemia reperfu-sion injury. Methods: Reversible middle cerebral artery occlusion(MCAO) was induced 1 h after ischemia. The rats used to determine the infarction region of cerebral injury were randomly allocated into 3 groups: MCAO-treatment group, MCAO-nontreatment group, and control group (no injury, no treatment). The rats used to calculate brain water content were randomly allocated into 2 groups: MCAO-treatment group and MCAO-nontreatment group. Infarction region was measured with TTC staining 1 d after ischemia. Brain water content was estimated 2 d after ischemia. Results: Compared with control group, the infarction region decreased (P<10. 01), and brain edema in MCAO-treatment group ameliorated(P<0. 01). Conclusion: Cycolsporin A has obvious protective effects on cerebral ischemia reperfusion injury in rats.