7种蛇毒小肽对临床分离耐（多）药结核分枝杆菌的体外活性研究

 目的检测7种从蛇毒分离的小肽是否对临床分离的耐药性结核分枝杆菌菌株具有活性。方法放射性方法检测蛇毒小肽对结核分枝杆菌的最小抑制浓度，细菌存活计数确证放射性方法的结果。结果7种蛇毒小肽对耐药性结核分枝杆菌菌株都有活性。其MIC值分别为(μg·mL_-1)：Opiophagus hannah 5.4，Naja atra 8.6，Bungarus fasciatus 6.4，Trimeresurus stejnegri 12.6，Protobothrops mucrosquamatus 11.8，Protobothrops jerdonii 7，Agksistrodon halys 4.2。结论这些结果是首次报道，为进一步设计和开发新来源的抗结核病新药提供了依据。

In Vitro activities of seven small peptides from snake venoms against clinical (multi)drug-resistant Mycobacterium tuberculosis isolates

OBJECTIVE To determine whether the 7 small peptides isolated from snake venoms possess activities against clinical isolated multidrug resistant strains of Mycobacterium tuberculosis.METHOD S The MICs of the small peptides were determined by radiometric,and confirmed by bacterial viable count.RESULTS All seven tested small peptides showed activities against the above strains.The MIC values were (unit:μg·mL_-1) Opiophagus hannah 5.4，Naja atra 8.6，Bungarus fasciatus 6.4，Trimeresurus stejnegri 12.6，Protobothrops mucrosquamatus 11.8，Protobothrops jerdonii 7，Agksistrodon halys 4.2,respectivly.CONCLUSION The results provided a basis for further developing novel anti-mycobacterial agents and improving treatment of tuberculosis.