厌氧菌数值鉴定系列培养基的制备和应用

本文报道使用国产材料及试剂研制出厌氧菌数值鉴定系列培养基,用国产气相色谱仪建立厌氧菌代谢终产物的检查方法,并以厌氧菌的数值鉴定原理、操作方法、药敏试验、厌氧菌的新种及厌氧菌常见属种编码等为内容编辑出《厌氧菌数值鉴定指南》,在我国首次建立厌氧菌的简易鉴定系统。
用常规厌氧菌鉴定法为对照,使用这个鉴定系统检查64株标准厌氧菌和277株从临床感染症分离到的厌氧菌。鉴定结果,属水平的鉴定符合率为96%,种水平的鉴定符合率为76%,与法国的API-20A的鉴定效果一致。经北京、长春、南昌等地的六个科研、生产及医疗单位考核验证,共同认为:这个厌氧菌鉴定系统操作简便,出报告结果的时间快,费用低廉,适合医学、兽医学、科研教学单位的中小型实验室使用。

A System of Numerical Identification of Anaerobic Bacteria Studies on Preparation & Use of the Culture Media

The paper presents the initial establishment in China an system for numeric l identification of anaerobic b cteria (NISA). The system consists of preparing serial culture media with n tional biochemicals for use in identification tests, utilizing Chinamade gas chromatography system in analysing the end metabolites, nd publishing a Manu l on (Theory & Practice of Numeric l System for Identifying An erobes) comparable to that of API-20A.
For evaluation of the proposed system, 64 strains of known anaerobes (type strains from CDC. VPI included) & 277 strains of anaerobic b cteria clinically isolated in China were identified with our system s compared with controls by utilizing the routine standard techniques for identifying anaerobes. A correlation of 96% was obt ined at the level of Genera and 76% as species. These results of identifying anaerobes compared favorably with those obtained from API-20A.
The results of evaluation prove that the proposed NISA in china possess the advantages of simplicity in application, rapidity in yielding laboratory report, inexpensive in cost, and suitable for clinical & laboratory use in assisting medical & veterinary diagnosis, and for te ching purposes in microbiology.