超声刺激对大鼠多皮层ECoG的调控

目的:为了进一步揭示超声刺激的神经调控机制以及超声刺激引起的刺激效应范围,将超声刺激经颅施加到大鼠前额叶皮层部位,同步记录和分析前额叶、顶叶及枕叶皮层的皮层电位(Electrocorticogram,ECoG)的变化特征.方法:在大鼠麻醉状态下,利用脑立体定位仪将超声探头定位在大鼠前额叶部位的正上方,刺激时间为20 m...

Regulation of electrocorticogram in multiple cortical layers of rats by ultrasound stimulation

Objective To further reveal the mechanisms of neuromodulation by ultrasound stimulation and the range of stimulatory effects caused by ultrasound stimulation,to apply ultrasound stimulation to the prefrontal cortex of rats for synchronously recording and analyzing the changes characteristics of electrocorticogram(ECoG)in the prefrontal,parietal and occipital cortices.Methods Under anesthesia,a stereotaxic apparatus was used to place the ultrasound transducer right above the prefrontal lobe of rats,and the stimulation duration was 20 min.Power spectral density(PSD),amplitude and interrelationship values were used as indicators to analyze and compare the total power and mean amplitude of ECoG as well as interrelationship values among cortices of pre-,mid-and post-ultrasonic stimulations.Results(1)Ultrasound stimulation increased the total PSD and mean amplitude of the ECoG of the prefrontal cortex(P<0.05).After the ultrasound stimulation stopped,the ECoG returned to the pre-stimulation level in a short time.(2)In the parietal and occipital cortices,ultrasound stimulation had no significant effect on the PSD and mean amplitude of ECoG and each frequency band.However,during the ultrasound stimulation,the average amplitude of the partial periods in the occipital cortex was obviously different from that before stimulation,which indicated that during the second half of the ultrasound stimulation,the range of the stimulatory effect caused by ultrasound stimulation was diffused to the occipital cortex.Conclusion Ultrasound stimulation can regulate the ECoG of local stimulation region,and the neuromodulation has the characteristics of promptness and rapid recovery.In general,the regulation effect of ultrasound stimulation on ECoG is limited.The effect of ultrasound stimulation on the ECoG of other cortical layers away from the stimulation sites remains unclear,and the effect is probably the subsequent neural network response caused by ultrasound stimulation.