| 性激素对喉癌细胞株HEp－2体外增殖的影响 |
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| 引用本文： | 赵小冬,肖雪媛,刘剑剀,郭晓峰,杨占泉,卜国铉. 性激素对喉癌细胞株HEp－2体外增殖的影响[J]. 中华耳鼻咽喉头颈外科杂志, 1994, 0(3) |
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| 作者姓名： | 赵小冬 肖雪媛 刘剑剀 郭晓峰 杨占泉 卜国铉 |
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| 作者单位： | 白求恩医科大学一院耳鼻咽喉科 |
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| 摘 要： | 本实验采用葡聚糖包被活性炭技术，观察小牛血清和葡聚糖包被活性炭处理的小牛血清对人喉癌细胞株细胞体外增殖的作用，及该细胞对睾酮，１７β－雌二醇和孕酮的剂量依赖性。结果表明该细胞是雄激素敏感型细胞，同时也是性激素非依赖型细胞。睾酮在１ｎｍｏｌ／Ｌ和１０ｎｍｏｌ／Ｌ浓度对该细胞的增殖有促进作用，孕酮和１７β－雌二醇无此作用。证明该细胞株是研究喉癌与性激素关系的适合的体外模型，为喉癌的内分泌治疗提供了参考依据。
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| 关 键 词： | 喉肿瘤；细胞株；性激素类 |
Effect of Sexual Hormones on Prollferation of Laryngeal Carcinoma Cell Line HEp-2in Vitro |
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| Zhao Xiaodong, Xiao Xueyuan, Liu Jiankai,et al.. Effect of Sexual Hormones on Prollferation of Laryngeal Carcinoma Cell Line HEp-2in Vitro[J]. Chinese journal of otorhinolaryngology head and neck surgery, 1994, 0(3) |
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| Authors: | Zhao Xiaodong Xiao Xueyuan Liu Jiankai et al. |
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| Affiliation: | Zhao Xiaodong, Xiao Xueyuan, Liu Jiankai, et al. |
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| Abstract: | In this study.we observed the effect of FCS and DCC FCS on the proliferation of HEp-2 cell in vitro with dextran-coated charcoal technique,and explored the dose-dependence of HEp-2 cell to testosterone,17 beta-estradiol and progesterone,The results showed that HEp-2 cell line is ndrogen-sensitive cell,and testosterone at concentration of 1 nmol / L and 10 nmol / L may stimulate the proliferation of HEp-2 cells,but 17 beta-estradiol and progesterone have no such effect.Tbe results suggested that HEp-2 cell line is a proper model in vitro for the study of laryngeal carcinoma and sexu- al hormones.This study offered the evidences of the endocrine therapy for laryngeal careinoma. |
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| Keywords: | Laryngeal neoplasms Cell Iine Sex hormones |
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