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Profiling celiac disease antibody repertoire |
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| Authors: | Sara D'Angelo Flavio Mignone Cecilia Deantonio Roberto Di Niro Roberta Bordoni Roberto Marzari Gianluca De Bellis Tarcisio Not Fortunato Ferrara Andrew Bradbury Claudio Santoro Daniele Sblattero |
| Affiliation: | 1. Department of Health Sciences and Interdisciplinary Research Center on Autoimmune Diseases (IRCAD), University of Eastern Piedmont “Amedeo Avogadro”, Novara, Italy;2. Dipartimento di Scienze e Innovazione Tecnologica, University of Eastern Piedmont “Amedeo Avogadro”, Alessandria, Italy;3. Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06510, USA;4. Institute of Biomedical Technologies, National Research Council (ITB CNR), Milan, Italy;5. Department of Life Sciences, University of Trieste, Trieste, Italy;6. Department of Medical Sciences University of Trieste, Institute of Child Health IRCCS “Burlo Garofolo”, Trieste, Italy;g Los Alamos National Laboratory, Los Alamos, NM, USA |
| Abstract: | The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research. |
| Keywords: | Celiac disease Autoantibody Autoantigen Protein microarray ORF-display libraries Next generation sequencing |
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