先天性巨结肠神经生长因子及神经生长因子受体基因表达

目的探讨神经生长因子(nerve growth factor，NGF)及其神经生长因子受体(nerve growth factor receptor，NGFR)对先天性巨结肠(Hirschsprungs disease，HD)的调控作用。方法采用RT-P(永技术检测新鲜标本正常对照肠管、痉挛段、移行段和扩张段各22例NGF和NGFR mRNA的表达，分析并统计其与临床病理特点之间的关系。结果22例正常对照肠管21例NGF mRNA表达阳性(95．5％)，20例NGFR mRNA表达阳性(90．9％)；痉挛段5例NGF mRNA表达阳性(22．7%)，6例NGFR mRNA表达阳性(27．3％)；移行段9例NGF mRNA表达阳性(49．9％)，7例NGFR mRNA表达阳性(31．8％)；扩张段18例NGF mRNA表达阳性(81．8%)，19例NGFR mRNA表达阳性(86．4％)。NGF和NGFR mRNA正常肠管与扩张段肠管中表达高度一致；痉挛段和移行段均为NGF mRNA和NGFR mRNA低表达。结论NGF和NGFR与HD病理形态有相关性；HD的痉挛段和移行段肠管NGF及NGFR表达异常，提示NGF和NGFR基因可能与HD的发生有关。

The expression of nerve growth factor and nerve growth factor receptor genes in Hirschsprung's disease

Objective To explore the expression of nerve growth factor (NGF) and nerve growth factor receptor (NGFR) genes in Hirschsprung's disease (HD).Methods The mRNA of NGF and NGFR in normal, spastic, transitional and dilated segment were detected by RT-PCR in 22 fresh samples of HD. The relationship between expression of NGF and NGFR genes and pathological changes in colons were analyzed.Results In normal control, NGF mRNA was positive in 21 out of 22 ( 95.5%), NGFR mRNA was positive in 20 ( 90.9%). Meanwhile, NGF mRNA was positive in 18 ( 81.8) and NGFR in 19 ( 86.4%) in dilated segment. However, positive rate of NGF mRNA was decreased to 9 in transitional zone ( 49.9%) and to 5 in spastic segment ( 22.7%). Similar to the expression of NGF gene, NGFR mRNA was reduced to 7 ( 31.8%) in transitional zone and 6 ( 27.7%) in spastic segments.Conclusions NGF and NGFR genes may play some roles in the genesis of HD.