| 颈交感阻滞对烧伤早期小鼠各器官IFIT1表达的影响 |
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| 引用本文： | 巩继平,陈玉培,郭晓姝,粟永萍. 颈交感阻滞对烧伤早期小鼠各器官IFIT1表达的影响[J]. 医学争鸣, 2009, 30(6): 485-488 |
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| 作者姓名： | 巩继平 陈玉培 郭晓姝 粟永萍 |
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| 作者单位： | 巩继平,GONG Ji-Ping(第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,刨伤、烧伤与复合伤国家重点实验室,重庆,400038;重庆医科大学第二临床医学院麻醉科,重庆,400010);陈玉培,CHEN Yu-Pei(重庆医科大学第二临床医学院麻醉科,重庆,400010);郭晓姝,GUO Xiao-Shu(重庆医科大学基础医学院生理学教研室,重庆,400016);粟永萍,SU Yong-Ping(第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,刨伤、烧伤与复合伤国家重点实验室,重庆,400038) |
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| 基金项目： | 国家自然科学基金海外学者合作研究项目,国家重点基础研究发展规划(973计划) |
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| 摘 要： | 目的：观察小鼠心、肝、脾、肺、肾等器官干扰素诱导蛋白IFIT1（Interferon—induced protein with tetratfieopetide repeats1）的表达情况,探讨颈交感神经阻滞（cervical sympathetic block,SB）对烧伤后早期小鼠各器官IFIT1表达状况的影响．方法：将雄性昆明小鼠18只随机分为正常对照组、烧伤组和颈交感神经阻滞（SB）治疗组、烧伤组和SB治疗组采用体表总面积（Total body surface area,TBSA）15％-20％Ⅲ度小鼠烧伤模型,SB治疗组于烧伤后即刻进行双侧颈交感神经阻滞,各组分别于4h后取心、肝、脾、肺、肾组织,提取RNA,以RT—PCR检测IFIT1表达情况．结果：RT—PCR显示IFIT1在各组的各器官均有表达,并存在差异．烧伤组IFIT1在心、肝、脾表达低于正常组,同时在肺、肾表达高于正常组,差异显著（P〈0．01）．SB治疗组IFIT1的表达均低于正常组（P〈0．01）;同时,SB治疗组IFIT1在心、肝、肺、肾的表达也低于烧伤组（P〈0．01）．另外,在各组内的不同器官的IFIT1表达量存在差异（P〈0．05）．结论：烧伤后早期小鼠各器官IFIT1表达有不同的变化,可能与不同器官在烧伤应激反应过程中损伤程度不同有关．SB可显著降低各器官IFIT1的表达．
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| 关 键 词： | IFIT1 颈丛 自主神经传导阻滞 烧伤 |
Effect of cervical sympathetic block on Interferon-induced protein IFIT1 levels in organs of burned mice during early stage |
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| GONG Ji-Ping,CHEN Yu-Pei,GUO Xiao-Shu,SU Yong-PingPLA Institute of Combined Injury,& State Key Laboratory of Trauma,Burns , Combined Injury,Third Military Medical University,Chongqing China. Effect of cervical sympathetic block on Interferon-induced protein IFIT1 levels in organs of burned mice during early stage[J]. Negative, 2009, 30(6): 485-488 |
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| Authors: | GONG Ji-Ping CHEN Yu-Pei GUO Xiao-Shu SU Yong-PingPLA Institute of Combined Injury & State Key Laboratory of Trauma Burns Combined Injury Third Military Medical University Chongqing China |
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| Affiliation: | GONG Ji-Ping1,2,CHEN Yu-Pei2,GUO Xiao-Shu3,SU Yong-Ping11PLA Institute of Combined Injury,& State Key Laboratory of Trauma,Burns and Combined Injury,Third Military Medical University,Chongqing 400038 China,2Department of Anesthesia,School of Second Clinical Medicine,Chongqing Medical University,Chongqing 400010,China,3Department of Physiology,School of Preclinical Medicine,Chongqing 400016 |
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| Abstract: | AIM:To observe the expression of IFIT1(Interferon-induced protein with tetratricopetide repeats 1)in the heart,liver,spleen,lungs,kidneys of mice and to detect the effect of cervical sympathetic block(SB)on IFIT1 of organs during the early stage in burned mice.METHOD:Eighteen male Kunming mice were divided randomly into control group,burned group and SB group.Burned group and SB group were inflicted with 15%-20% TBSA(Total body surface area)full thickness burn injury and SB group was treated immediately by ... |
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| Keywords: | IFIT1 |
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