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HPLC法测定六味地黄片中丹皮酚的含量
引用本文:邓禄新. HPLC法测定六味地黄片中丹皮酚的含量[J]. 内蒙古中医药, 2014, 0(13): 102-103
作者姓名:邓禄新
作者单位:湖北中医药大学附属襄阳市中医医院药学部,湖北襄阳441000
摘    要:
目的 :利用HPLC法测定六味地黄片中丹皮酚的含量;方法:流动相:甲醇-水(70∶30),色谱柱:Diamonsil-C18(200×4.6mm,5μm),检测波长:274nm,流速:1.0mL·min-1,理论塔板数计算不低于3500;结果丹皮酚在4.364~26.184μg范围内线性关系良好,平均回收率为102.3%,RSD为0.73%;结论该方法比《卫生部药品标准中药成方制剂》第八册所示的用紫外分光光度法测定六味地黄片含量,方便、简单、灵敏度高、准确,可用于六味地黄片中丹皮酚的含量测定。

关 键 词:六味地黄片  丹皮酚  高效液相色谱法

Determination of Paeonol in Liuweidihuang Pills by HPLC
DENG Luxin. Determination of Paeonol in Liuweidihuang Pills by HPLC[J]. Nei Mongol Journal of Traditional Chinese Medicine, 2014, 0(13): 102-103
Authors:DENG Luxin
Affiliation:DENG Luxin (Dept.of Pharmacy, Hubei Traditional Chinese Medicine University Affiliated Hospital of Xiangyang Traditional Chinese Medicine hospital, Hubei Xiangyang 441000, China)
Abstract:
Objective:To develop HPLC method to assay liuweidihuang tabloit.;Methods:Separation was performed on a Diamonsil column(200×4.6mm,5μm).The mobile phase consist of methanol –water(70:30,v/v).The UV detection was set at 274nm,the flow rate was 1.0 mL· min-1;Resufs:Paeonol had a good liner relation in the range of 4.364—26.184ug,the average of the liuweidihuang pill was 102.3% and the RSD was 0.73%;Conclution:The method was sensitive,simple and rapid and it can be used to assay the paeonol content of liuweidihuang pills.
Keywords:Liuweidihuang pills  paeonol  HPLC
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