GW003对骨髓细胞体外形成粒-巨噬细胞集落能力的影响

本研究旨在观察重组人血清白蛋白-粒细胞集落刺激因子融合蛋白（GW003）对骨髓造血细胞体外形成粒系集落能力的影响。分别抽取正常恒河猴、缓解期和化疗中pLLL患者骨髓并分离有核细胞，分别加入系列浓度的GW003、吉粒芬、G-CSF突变体，在培养12d后计数粒-巨噬细胞系集落（CFU-GM）。结果表明，GW003具有提高正常恒河猴骨髓有核细胞体外形成CFU-GM的能力，效果明显优于相同摩尔浓度的吉粒芬 及G-CSF突变体；在-定的浓度范围内，GW003浓度与恒河猴骨髓细胞形成CFU-GM的数量呈明显的剂量效应关系（r=R2=0．965，P=0．003）；GW003能提高ALL患者骨髓细胞粒系集落体外形成能力，对化疗中患者的效果更为显著，可有效缓解化疗引起的骨髓抑制。结论：GW003可显著提高骨髓细胞体外形成粒细胞集落的能力，可有效缓解骨髓抑制。

Effect of GW003 on the Granulocyte Mecrophage Colony Formation Ability of Bone Marrow Cells In Vitro

The aim of this study was to investigate the effect of GW003 on the ability of granulocyte colony forming in vitro of bone marrow cells. The bone marrow samples was collected from normal rhesus, the patients with leukemia in stages of remission and chemotherapy respectively, and the nucleated cells were separated and cultrured for 12 days after addition of different concentrations of GW003 or rhG-CSF, or G-CSF mutant. Then the amount of colony-forming unit- granulocyte-macrophage was counted. The results indicated that GW003 could enhance the abilidy of bone marrow nucleated cells of rhesus to formin CFU-GM in vitro, and its effect was much better than that of rhG-CSF or G-CSF mutant at the same concentration. The GW003 showed dose-response relationship to CFU-GM level（ r = R2 = 0. 965, P =0. 003, in a certain concentration）, the GW003 also could enhance CFU-GM formation of marrow nucleated cells in leukemic patients, especially for patients receiving chemotherapy. The GW003 could relieve the marrow suppression caused by chemotherapy significantly. It is concluded that the GW003 can significantly improve the ability of bone marrow cells to form granulocyte colony in vitro as well as effectively alleviate bone marrow suppression.