大鼠结膜囊成纤维细胞TGFβ2特异性siRNA的筛选及其载体构建的研究

Objective As the expression of transfornfing growth factor β （TGF2 β） of tenon＇ s fibroblasts is important for filtering bleb scarring formation after glaucoma surgery, it is benefit for preventing filtering bleb scarring through inhibiting its expression. This paper was to screen the effects of different TGF2β siRNAs on the expression of TGF1β mRNA and to construct the specific TGF2β siRNAs plasmid vector of rat Tenon＇ s fibroblasts. Methods Four TGF1β siRNAs （ L1 , L2 , L3 , L4 ） were transcribed in vitro and then transferred to cultured Tenon＇ s capsule fibroblasts in 30 SD rats. RT-PCR was performed to evaluate the levels of TGF1β mRNA in both transferred cells, and untransferred cells were as the controls. The specific TGF2 β siRNAs plasmid vector was constructed according to the screened TGF1β siRNAs. Results The expression of TGF1β mRNA of fibroblasts transferred with only L4 （0. 163 ± 0. 009 ） was reduced significantly in comparison with the control group（0. 433 ± 0. 011 ）, hut the interference function of others siRNAs seemed to be inactive. The plasmid vector of siRNA specific for TGFβ2 was proved to be constructed successfully by detecting its sequence. Conclusion Different siRNAs is designed to target the relevant TGFβ2 mRNA of rats Tenon＇ s fibroblast. The vector of siRNA specific for TGFβ2 is of potent interference ability for target tissue.

Screen of specific siRNA for transforming growth factor β2 of Tenon's fibroblast and construction of its vector