高效液相色谱法测定人血浆中视黄醇和生育酚

本文介绍用高效液相色谱和内标法同时测定人血浆中视黄醇(维生素A)和α-及γ+β生育酚(维生素E)。用C_(18)为分析柱,前加二预柱。流动相为甲醇和水(98:2)。紫外检测器之检测波长为300nm。色谱分析在12min内完成。用微处理机C-RIB之计算程序,内标二点法以及峰面积比值为单位计算结果。本方法回收率的均值,视黄醇为93.9％,α-生育酚为106.9％;变异系数(CV％),视黄醇为5.2％,α-生育醇为6.3％,γ-生育酚为4.8％。比较了用混合测定维生素A与E的结果与单样测定的结果,两者很一致。用混合样测定结果做为评价指标可以节省血样和大量人力、物力。在我国65个县130个农村点中取男女血样各3250份。血浆中维生素A和E的水平以平均值±SD(μg/dl)表示,我国人血浆视黄醇含量,男性为52±9.2(39～79),女性为41±6.8(30～54);α-生育酚,男性为703±108.7 (586～950),女性为735±101.1(594～1075),总生育酚,男性为750±124,女性为776±104。

MEASUREMENT OF PLASMA VITAMIN A AND E IN CHINESE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

This paper described a microprocedure for the simultaneous determination of retinol (Vitamin A), alpha and gamma tocopherol (Vitamin E) in plasma by high performance liquid chromatography. A reversed-phase C18 column with precolumn was used. The mobile phase was water in methanol (2:98) and the flow rate was 1.8ml/min. An ultraviolet detector with 300 nm was used. The chromatogram was completed in 12 min and the retinol, alpha and gamma tocopherol were quantitated by the peak area ratio and weight ratio using benzo (e) pyrene as an internal standard. The mean recovery of retinol and alpha tocopherol were 93.9% and 106.9% respectively. The coefficient of variation (CV%) were 5.2 for retinol, 4.8 for gamma tocopherol and 6.3 for alpha tocopherol. In order to assess the possibility of measuring pooled samples instead of individual samples, the value of individuals was compared with the value of the pooled sample. The results showed that the pool values and the mean ± SD of the 25 individuals compared very favourably.6500 blood samples were collected from both sexes and 130 communities of 65 counties. The plasma retinol and tocopherols were analyzed. The average plasma retinol value (X±SD,μg/dl) was 52 ±9.2 for male and 41 ±6.8 for female. The average plasma alpha tocopherol value (X±SD) was 703±108 for male and 735±101 for female. The mean ± SD (μg/dl) of total vitamin E were 750 ± 124 and 776 ±104 respectively for male and female.