藿酮胶囊诱导人体血管平滑肌细胞凋亡的实验研究

目的：从细胞凋亡水平研究藿酮胶囊抗动脉样硬化的作用机理。方法：实验培养人胚胎胸主动脉平滑肌细胞,用白细胞介素－１（ＩＬ－１）刺激平滑肌细胞建立血管平滑肌细胞（ｖａｓｃｕｌａｒ ｓｍｏｏｔｈ ｍｕｓｃｌｅ ｃｅｌｌ,ＶＳＭＣ）增生模型,从细胞形态学,ＤＮＡ凝胶电泳,流式细胞术３个方面观察用藿酮胶囊诱导增生的ＶＳＭＣ凋亡现象。结果：透射电镜观察发现：藿酮胶囊处理的ＶＳＭＣ出现细胞皱缩,染色质凝集后细胞

Experimental Study of Vascular Smooth Muscle Cell Apoptosis Induced by Huotong Capsule

OBJECTIVE: To explore the anti-atherogenic mechanism of Huotong Capsule (HTC) on vascular smooth muscle cell (VSMC) apoptosis. METHODS: VSMC proliferation model of cultured human embryonal thoracic aorta was established by stimulating VSMC with interleukin-1 (IL-1) and HTC was applied to observe the apoptosis inducing effect by transmission electron microscopy, DNA gel electrophoresis and flow cytometry. RESULTS: Typical apoptotic cellular changes were found after HTC treatment, the VSMC shrinked, the nuclei disappeared after chromatin agglutination, cellular membrane projected and wrapped the organelle to form apoptotic body. DNA gel electrophoresis showed "ladder" strand of DNA, a special phenomenon of cellular apoptosis. And an Ap peak of degraded DNA could be seen by flow cytometry, which widened and heightened when the concentration of HTC increased. The above-mentioned changes were not found in the blank control and untreated model group. CONCLUSION: HTC could induce apoptosis of human proliferated VSMC.