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| RP-HPLC法测定中药挥发油微乳中甲基丁香酚的含量 | |
| 引用本文： | 杨蒙蒙,张婷婷,刘新友,覃华,张琰. RP-HPLC法测定中药挥发油微乳中甲基丁香酚的含量[J]. 中国医院用药评价与分析, 2011, 0(6): 545-546 |
| 作者姓名： | 杨蒙蒙 张婷婷 刘新友 覃华 张琰 |
| 作者单位： | 第四军医大学唐都医院药学部; |
| 摘 要： | 目的:建立中药挥发油微乳中甲基丁香酚的含量测定方法。方法:采用反相高效液相色谱法测定,色谱柱:Hypersil ODS2柱(4.6 mm×150 mm,5μm);流动相∶甲醇-水(80∶20);检测波长:275 nm;柱温:25℃;流速:1.0 mL.min-1。结果:甲基丁香酚在12.42～49.68μg范围内与峰面积线性关系良好,相关系数为0.999 1,平均加样回收率为98.51%,RSD为1.22%。结论:该法操作简便、准确,可作为中药挥发油微乳的质量控制方法之一。 |
| 关 键 词： | RP-HPLC法 中药挥发油微乳 甲基丁香酚 |
Determination of Methyleugenol in Microemulsion Prepared from Volatile Oil of Chinese Herbal Medicine by RP-HPLC |
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| YANG Meng-meng,ZHANG Ting-ting,LIU Xin-you,QIN Hua,ZHANG Yan. Determination of Methyleugenol in Microemulsion Prepared from Volatile Oil of Chinese Herbal Medicine by RP-HPLC[J]. Evaluation and Analysis of Drug-Use in Hospital of China, 2011, 0(6): 545-546 | |
| Authors: | YANG Meng-meng ZHANG Ting-ting LIU Xin-you QIN Hua ZHANG Yan |
| Affiliation: | YANG Meng-meng,ZHANG Ting-ting,LIU Xin-you,QIN Hua,ZHANG Yan(Dept.of Pharmacy,Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038,China) |
| Abstract: | OBJECTIVE：To establish a method for determination of methyleugenol in the microemulsion prepared from volatile oil of Chinese herbal medicine.METHODS：By a RP-HPLC in which Hypersil ODS2 column（4.6 mm×150 mm,5μm） was used with a mobile phase consisting of methanol and water（80∶20） at a flow rate of 1 mL·min-1.The column temperature was set at 25℃ and the detection wavelength was set at 275 nm.RESULTS：Methyleugenol had good linearity in the range of 12.42-49.68 μg（r=0.999 1）.The average recovery rate was 98.51%（RSD=1.22%）.CONCLUSION：The method is simple and accurate and it can be used as one method for quality control of microemulsion prepared from volatile oil of Chinese herbal medicine |
| Keywords: | RP-HPLC Volatile oil Chinese herbal medicine Methyleugenol |
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