淋巴组织表达受体在食管鳞状细胞癌中表达水平与临床病理参数及预后的关系

目的 观察淋巴组织表达受体(receptor expressed in lymphoid tissue,RELT)在食管鳞状细胞癌组织中的表达水平,探讨其与临床病理参数及预后的关系.方法 食管鳞状细胞癌患者83例,取手术切除的鳞状细胞癌组织和癌旁正常组织标本,采用实时荧光定量PCR法检测2种组织RELT mRNA相对表...

Relationships of receptor expressed in lymphoid tissues in esophageal squamous cell carcinoma with clinicopathological parameters and prognosis

Objective To observe the expression level of receptor expressed in lymphoid tissues(RELT) in esophageal squamous cell carcinoma tissues, and to investigate its relationships with clinicopathological parameters and prognosis. Methods Real-time fluorescence quantitative PCR was used to detect the relative expression of RELT mRNA in resected squamous cell carcinoma cancer tissues and adjacent normal tissues from 83 cases of esophageal squamous cell carcinoma. The positive rate of RELT protein was detected by immunohistochemistry. The relative expression of RELT protein was detected by Western blot. According to the median relative expression of RELT mRNA, 83 patients were divided into low-expression group(n=42) and high-expression group(n=41). The clinical data of two groups were compared, and the disease-free survival time was compared by Kaplan-Meier survival analysis between two groups. The COX proportional hazard was used to analyze the risk factors of poor prognosis of esophageal squamous cell carcinoma. Results The relative expressions of RELT mRNA(8.022±2.301) and RELT protein(0.867±0.085) as well as the positive rate of RELT protein(78.31%) in esophageal squamous cell carcinoma tissues were higher than those in adjacent normal tissues(3.080±0.754, 0.634±0.123, 16.87%)(P<0.05). The percentages of patients <60 years(68.29%), in T₃-T₄ stage(75.61%), in TNM stage ⅡB-Ⅲ(68.29%), with lymph node metastasis(58.54%) and vascular invasion(63.41%) in high-expression group were higher than those in low-expression group(38.10%, 45.24%, 42.86%, 26.19%, 28.57%)(P<0.05). The median disease-free survival time was shorter in high-expression group(16 months) than that in low-expression group(>30 months)(P<0.05). The relative expression of RELT mRNA ≥8.009(HR=2.120, 95%CI: 1.102-4.078, P=0.024), and TNM stageⅡB-Ⅲ(HR=36.348,95%CI:2.202-599.976,P=0.012)were the risk factors of poor prognosis of esophageal squamous cell carcinoma.Conclusion The high expression of RELT in patients with esophageal squamous cell carcinoma is associated with poor prognosis,and may be involved in the occurrence and development of esophageal squamous cell carcinoma.