短暂缺氧、血清剥夺再灌流后神经细胞顿抑现象的研究

目的　研究短暂缺氧、血清剥夺复氧复注血清 (再灌流 )后有无神经细胞顿抑现象存在及可能的发生机制。方法　将PC12细胞随机分为正常对照组和缺氧组 ,每组根据不同的再灌流时间点又分为 3个亚组 (缺氧组分别为缺氧 15min再灌流 1h组、3h组、6h组 ,正常对照组各亚组的时间点与缺氧组相对应 )。测定短暂缺氧血清剥夺再灌流后不同时间点的三磷酸腺苷含量、线粒体膜电位和细胞活性。结果　与正常对照组相比 ,缺氧血清剥夺 15min再灌流 1h后的三磷酸腺苷含量、线粒体膜电位、细胞活性显著降低 ,差异具有显著性意义 (P <0 .0 5 ) ;缺氧血清剥夺 15min再灌流 6h后基本恢复至正常水平 ,差异无显著性意义 (P >0 .0 5 )。结论　短暂缺氧血清剥夺再灌流后 ,存在低能量状态 ,且可完全恢复 ,表明可能存在神经细胞顿抑现象 ,其原因可能与缺氧血清剥夺再灌流后线粒体呼吸链上的琥珀酸脱氢酶等活性下降及线粒体的膜电位变化等有关。

A study of the stunning phenomenon of nerve cells after transient anoxia-serum deprivation / reperfusion

Objective To identify whether there is nerve cells stunning after transient anoxia-serum deprivation / reperfusion and to explore the probable underlying mechanism of the phenomenon. Methods The PC12 cells were randomly divided into a control group and a anoxia group, the two groups were divided into 3 subgroups, respectively, according to time of reperfusion after 15 minutes of anoxia-serum deprivation. The content of adenosine triphosphate, mitochondria membrane potential and cell activity were determined at different time points after transient anoxia-serum deprivation / reperfusion. Results The content of adenosine triphosphate , mitochondria membrane potential and cell activity after 15 minutes of anoxia-serum deprivation plus 1 hour of reperfusion in the anoxia group were significantly lower than those in the control group( P <0.05)and they recovered to the level of the control group after 6 hours of reperfusion. Conclusion There was lower energy status after transient anoxia-serum deprivation / reperfusion, the lower energy status can recover to the normal level. The result showed that there might be nerve cells stunning. This phenomenon probably resulted from lower succinate dehydrogenase activity in the respiratory chain of the cells and the changes of mitochondria membrane potential.