细胞内钙离子螯合剂抑制HSV-1诱导Hela细胞的凋亡作用

目的：探讨HSV-1在诱导Hela细胞凋亡中，细胞内游离钙浓度的变化及钙离子螯合剂对HSV-1诱导Hela细胞凋亡的抑制作用。方法：HSV-1感染Hela细胞后，用扫描电镜及透射电镜观察凋亡情况。用荧光探针标记细胞内游离Ca^2 ，在不同时间观察细胞内游离Ca^2 浓度的变化。结果：HSV-1感染Hela细胞后，出现了典型的凋亡形态学变化。细胞内游离Ca^2 浓度在HSV-1感染Hela细胞后12h达高峰；典型的凋亡形态学变化出现在HSV-1感染Hela细胞后24h。细胞内Ca^2 螯合剂能显著抑制HSV-1诱导的Hela细胞凋亡。结论：细胞内游离Ca^2 在HSV-1诱导的Hela细胞凋亡过程中，具有重要作用，此实验结果为临床治疗相关疾病提供了有用的线索。

Inhibition of intracellular Ca2+-chelating agent on Hela cell apoptosis induced by HSV-1

AIM: To explore the variation of intracellular free Ca 2+ concentration during Hela cell apoptosis induced by HSV 1 and inhibition of Ca 2+ chelating agent on the apoptosis. METHODS: Apoptosis of the Hela cells infected by HSV 1 was observed under scanning electron microscope (SEM) and transmission electron microscope (TEM), and the variation of intracellular free Ca 2+ concentration, labeled with fluorescent probe was observed under Fluorescence microscope at a series of timepoints. RESULTS: The Hela cells infected by HSV 1 showed typical apoptotic morphological changes. Free Ca 2+ concentration increased and reached peak at the 12 hours after HSV 1 infection. Characteristic morphological features of apoptosis occured at the 24 hours after infection. This apoptosis can be inhibited by intracellular Ca 2+ chelating agent. CONCLUSION: Intracellular free Ca 2+ might play an important role in Hela cell apoptosis induced by HSV 1, which would provide useful clues for clinical treatment of associated diseases.