大鼠肝硬变门脉高压形成中和门奇静脉断流术后血甘氨胆酸和胰高糖素变化的研究

应用四氯化碳诱发建立大鼠肝硬变模型,以观察大鼠肝硬变形成过程中和行门奇静脉断流术后门静脉压力（ＰＶＰ）、门脉及外周静脉血中甘氨胆酸（ＣＧＡ）和胰高糖素（ＰＧ）的变化。结果：肝硬变早期ＰＶＰ及外周血ＣＧＡ和ＰＧ开始增高,门脉血ＣＧＡ降低,而ＰＧ增高,且随肝硬变程度加重,以上各指标的改变更明显。断流术后肝硬变大鼠外周血ＣＧＡ增高,ＰＶＰ、门脉血ＣＧＡ及外周和门脉血ＰＧ均无明显变化。正常大鼠以上指标均无改变。本实验结果提示：外周血ＣＧＡ水平既能早期准确反映肝细胞损伤,从而早期诊断肝硬变,还能判断肝硬变的严重程度及其预后;肝硬变时,外周和门脉血ＰＧ水平与ＰＶＰ的高低密切相关;断流术能维持ＰＶＰ及门脉血ＰＧ水平,对正常大鼠肝功能影响小,但对肝硬变大鼠的肝细胞有损害。

CHANGES OF GLYCOCHOLIC ACID AND PANCREATIC GLUCAGON IN PORTAL AND PERIPHERAL VENOUS BLOOD OF RATS WITH EXPERIMENTAL CIRRHOTIC PORTAL HYPERTENSION AND AFTER PORTAL-AZYGOUS DEVASCULARIZATIOIN

To investigate the change of the portal venous pressure (PVP), conjugated glycocholic acid (CGA) and pancreatic glucagon (PG) concentration in rats peripheral and portal venous blood in the course of experimental liver cirrhosis induced with carbon tetrachloride and to investigate the mentioned changes after portal azygous devascularization. The authors found that in the early stage of cirrhosis the PVP and the concentration of CGA and PG in peripheral venous blood were increased markedly, CGA in portal vein was decreased and PG in portal vein was increased in early stage of liver cirrhosis.With the extent of liver cirrhosis the indexes above changed more markedly. After portal azygous devascularization the concentration of CGA in peripheral vein in the cirrhotic rats was increased but PVP, the concentration of CGA in portal vein and PG in peripheral and portal vein did not change.There was no change in nornal rats. The results suggest that the variation in CGA in peripheral vein can accurately reflect the degree of damage to liver cells, thus making the diagnosis of liver cirrhosis earlier and judging the degree and prognosis of liver cirrhosis.The concentration of PG in portal venous and peripheral vein relate to PVP in liver cirrhosis.Portal azygous devascularization can maintain PVP and PG in portal vein and do not affect liver function of the control rats but it can damage liver cell in cirrhotic rats.