弥漫型胃癌组织中增殖细胞核抗原的表达

目的：研究弥漫型胃癌增殖细胞核抗原（PCNA）的表达与肿瘤浸润,淋巴结转移的关系。方法：采用免疫组化法,对11例弥漫型胃癌组织中PCNA的表达水平进行检测,结果：112例弥漫型胃癌中96例（85．7％）PCNA阳性,粘膜及粘膜下肿瘤PCNA阳性率（28．6％）明显低于浆浆膜外肿瘤（93．0％）,（P＜0．05）,淋巴结转移组PCNA阳性率（82．0％）明显高于无淋巴结转移组（34．8％）,淋巴结转移≥10枚淋巴结组PCNA阳性率（94．1％）明显高于＜10枚淋巴结组（42．9％）（P＜0．05）。结论L对胃癌组织进行PCNA蛋白的检测,有助于预测胃癌进展程度和诊断淋巴结转移,以及评估胃癌的预后。

Expression of PCNA in diffuse gastric carcinoma

Aim: To study the correlation of expression of proliferating cell nuclear antigen (PCNA) with infiltration of the tumor and lymph node metastasis in diffuse gastric carcinoma. Methods: The expression of PCNA in 112 cases of diffuse gastric carcinomas was detected by immunohistochemistry (SP). Results;Of the 112 cases,96 cases(85. 7% ) showed PCNA positive expression. The PCNA positive rate in intramucosal and submcuosal tumors (28.6% ) was significantly lower than that in inserosal and extraserosal tumors (93.0%) (P <0. 05). The PCNA positive rate in the group with lymph node metastasis (82.0% ) was significantly higher than that without (34. 8% ) (P <0.05). The PCNA positive rate (94. 1% ) of lymph nodes metastasis equal to or more than 10,was significantly higher than that of lymph node metastasis less than 10 (42.9% ) (P <0.05). Conclusion:It is concluded that the detection of the PCNA protein by SP might be helpful in assessing the tumor progression, diagnosing the lymph nodes metastasis, and predicting the prognosis of the gastric carcinoma.