Hp培养滤液诱导下抑制hTERT基因对SGC27901细胞bcl-2蛋白表达的影响

目的：探讨bcl-2表达与端粒酶活性二者之间的调控关系，了解Hp的致癌机制。方法：在Hp培养滤液诱导前后，采用流式细胞仪检测抑制hTERT基因之后SGC7901胃癌细胞bcl-2蛋白的表达。结果：对照组SGC7901胃癌细胞24h、36h和48h表达bcl-2蛋白的阳性细胞率和荧光指数显著低于经Hp培养滤液诱导的SGC7901胃癌细胞组（P〈0．05）。在Hp滤液诱导下，抑制hTERT基因的SGC791胃癌细胞24h、36h和48h表达bcl-2蛋白的阳性细胞率和荧光指数与SGC7901胃癌细胞无显著差异（P〉0．05）。结论：Hp感染可以促进bcl-2蛋白表达，这可能是Hp感染致胃癌的重要机制之一。端粒酶活化不参与调控bcl-2蛋白表达。

Effects of H. pylori incubating filtrate on expression of bcl - 2 protein in human 7901 gastric carcinoma cells transferred antisense hTERT

Objective: To explore the relationship between the activity of telomerase and the expression of bcl-2 protein and to study the carcinogenic mechanism of H.pylori.Methods: The expression of bcl-2 protein in SGC7901 cells transferred antisense hTERT was detected by flow cytometryanalysator(FCM) in the presence of low concentration of H.pylori incubating filtrate.Results: The rate of cells and the fluorescent index of expressing bcl-2 protein in the control SGC7901 cells were significantly lower than those in SGC7901 cells culture with H.pylori filtrate in 24,36 and 48 h(P<0.05).It was not remarkable the differences of the rate of cells and fluorescent index of expressing bcl-2 protein between SGC7901 cells and SGC7901 cells transferred antisense hTERT both cultured with H.pylori filtrate in 24,36 and 48 h(P>0.05).Conclusion: H.pylori infection could promote the expression of bcl-2 protein in the SGC7901 cell,which may be an important cancerigenic mechanism of H.pylori.Telomerase does not take part in the regulation of the expression of bcl-2 protein.