单次低剂量丙泊酚联合肢体缺血后处理对大鼠脑缺血再灌注损伤的影响

目的:探讨单次低剂量丙泊酚联合肢体缺血后处理(LIPC)对大鼠脑缺血再灌注损伤的影响。方法:100只雄性SD大鼠分为5组:假手术组、模型组、丙泊酚组、LIPC组和联合治疗组(丙泊酚+LIPC),每组20只。采用线栓闭塞大脑中动脉法建立局灶性脑缺血再灌注模型。测定神经功能缺陷评分,脑梗死面积,MDA含量,SOD、GSH-Px活性以及IL-6、TNF-α水平。结果:5组大鼠以上指标相比,差异均有统计学意义(P<0.001);低剂量丙泊酚和LIPC均能改善脑缺血再灌注大鼠神经功能缺损症状,减少脑梗死面积,降低IL-6水平、TNF-α水平及MDA含量,抑制SOD、GSH-Px活性的下降,而丙泊酚与LIPC联合应用效果更为明显(P<0.05)。结论:低剂量丙泊酚联合LIPC可通过抑制氧化应激和炎症反应减轻脑缺血再灌注损伤,两者合用具有协同效果。

Effects of single low dose propofol with limb ischemia postconditioning on cerebral ischemia/reperfusion injury in rats

Aim:To investigate the effects of single low dose propofol with limb ischemia postconditioning(LIPC) on cerebral ischemia/reperfusion injury in rats.Methods:A total of 100 male SD rats were randomly allocated into five groups:sham group,model group,propofol group,LIPC group and combined treatment group(propofol plus LIPC),20 in each group.The focal cerebral ischemia/reperfusion rat model was set up by using the middle cerebral artery occlusion method.The neurological deficit score,infarct size,malondialdehyde(MDA) content,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities,interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels were detected.Results:There were significant differences in the above parameters among the 5 groups(P<0.001);low dose propofol and LIPC both could improve neurological deficit symptoms,reduce infarct size,decrease IL-6,TNF-α level and MDA content,inhibit the decrease of SOD and GSH-Px activities in cerebral ischemia/reperfusion rats,while the effect of propofol with LIPC combinated treatment was more obvious(P<0.05).Conclusion:Low dose propofol with LIPC is able to reduce cerebral ischemia/reperfusion injury by inhibiting oxidative stress and inflammation reaction,and combined treatment displays synergistic effect.