黄蓝颗粒对风疹病毒抑制作用的临床和实验研究

目的 探讨黄蓝颗粒对风疹病毒抑制作用的疗效及其作用机理。 方法 将RuV IgM阳性患者60例随机分为2组, 每组均为30例。治疗组给予黄蓝颗粒（黄芪30 g板蓝根30 g贯众30 g，武汉市中西医结合医院制剂室提供）口服，每天1剂,分两次温开水冲服。对照组给予利巴韦林(0.1 g/片) 口服,每次0.2 g,每天3次。两组均以20天为1个疗程。观察Ru-IgM转阴率，并检测血清白细胞介素（IL-2）、肿瘤坏死因子（TNF-α）水平。体外实验研究采用细胞病变抑制法在非洲绿猴肾（Vero）细胞培养上测定黄蓝颗粒对RuV Gos株的抑制作用。结果 临床研究表明，治疗组和对照组1个疗程转阴率（分别为86.7%,63.3%）比较,差异有统计学意义(P<0.05）；2个疗程转阴率（分别为100%,86.7%）比较,差异无统计学意义 (P>0.05)。RuV-IgM阳性患者IL-2较正常组显著下降，而TNF-α则显著升高；黄蓝颗粒治疗后IL 2和TNF-α恢复至正常水平。体外实验研究发现，黄蓝颗粒对RuV所致细胞病变有明显的抑制作用。结论 黄蓝颗粒在体内外对RuV均有显著的抑制作用，而且能提高机体免疫力，是中医药治疗风疹病毒感染的有效药物。

Clinical and Experimental Study on Effects of Huanglan Granule in Inhibiting Rubella Virus

OBJECTIVE: To explore the therapeutic effect and acting mechanism of Huanglan Granule (HLG) on rubella virus (RuV). METHODS: Sixty patients with positive RuV-IgM were randomly assigned to two groups equally, the treatment group was medicated by HLG (one dosage per day, containing milkvetch root, isatis root and basket fern, each 30 g), while the control group by ribavirin (0.2 g, three times per day) for 20 days. The negative conversion rate of RuV-IgM and the serum levels of interleukin-2 (IL-2) and tumor necrosis factor alpha (TNF-alpha) were observed before and after treatment. Moreover, the in vitro inhibitory activity of HLG against RuV Gos line on cultured Vero cells was determined by cytopathic inhibition method. RESULTS: The difference of negative conversion rate between the two groups after one course treatment was significant (86.7% vs 63.3%, P <0.05). However, it turned to insignificant after two courses of treatment (100% vs 86.7%, P >0.05). The serum level of IL-2 was lower and TNF-alpha was higher significantly in patients with positive RuV-IgM as compared with the normal range, and the two indexes returned to the normal range rapidly after HLG treatment. In vitro study showed that the inhibitory effect of HLG on RuV caused cellular change was evident. CONCLUSION: HLG has obvious inhibitory effect on RuV, both in vitro and in vivo, it can also raise the immunity of organism and thus it serves as a safe and effective Chinese medicine for treatment of active RuV infection.