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| 变形链球菌表面蛋白真核表达载体pcDNA3-PAc的构建Ⅲ.变形链球菌表面蛋白PAc编码基因pac的体外扩增 | |
| 引用本文： | 刘建国,刘天佳,周学东,杨德琴,张义正,王海燕.变形链球菌表面蛋白真核表达载体pcDNA3-PAc的构建Ⅲ.变形链球菌表面蛋白PAc编码基因pac的体外扩增[J].贵州医药,2003,27(8):679-681. |
| 作者姓名： | 刘建国 刘天佳 周学东 杨德琴 张义正 王海燕 |
| 作者单位： | 1. 遵义医学院附属口腔医院,563003 2. 四川大学华西口腔医学院 3. 四川大学生命学院 |
| 基金项目： | 国家自然科学基金资助项目 (编号 3 9770 797) |
| 摘 要： | 目的 获取完整的变形链球茵表面蛋白PAc的编码基因pac。方法 建立扩增长片段目的基因的PCR反应体系，根据文献报道的pac核苷酸序列设计引物，使用通用缓冲液和高纯度的质粒pPC4l DNA为模板，经PCR扩增pac基因。结果 扩增产物经琼脂糖电泳和分子量鉴定，为单一的4．7kb条带。结论 成功地从质粒pPC4l DNA模板上扩增到全长4．7kb的完整pac基因，为下一步的防龋核酸疫苗的研究提供了物质基础。 |
| 关 键 词： | 变形链球菌 pcDNA3-PAc 基因克隆 聚合酶链反应 |
Construction of streptococcus mutans surface protein antigen eukaryotic plasmid expression vector Ⅲ. Amplification of the pac gene DNA fragment of srteptococcus mutans surface protein antigen by polymerase chain reaction |
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| Liu Jianguo,Liu Tianjia,Zhou Xuedong,et al..Construction of streptococcus mutans surface protein antigen eukaryotic plasmid expression vector Ⅲ. Amplification of the pac gene DNA fragment of srteptococcus mutans surface protein antigen by polymerase chain reaction[J].Guizhou Medical Journal,2003,27(8):679-681. | |
| Authors: | Liu Jianguo Liu Tianjia Zhou Xuedong |
| Institution: | Liu Jianguo,Liu Tianjia,Zhou Xuedong,et al. The Affiliated Hospital of Stomatology,Zunyi Medical College,Zunyi 563003 |
| Abstract: | Objective To obtain the complete pac gene DNA fragment of streptococcus mutans surface protein antigen.Methods Used a long-distance polymerase chain reaction (PCR) assay with plasmid pPC41 as template and primers designed by ourselves to amplify pac gene. Results A single 4.7kb DNA band was produced and estimated by gel electrophoresis. Conclusion The 4.7kb gene DNA fragment was successfully obtained from plasmid pPC41 by PCR, which could be applied in the study of nucleic acid vaccines of dental caries. |
| Keywords: | Streptococcus mutans Vaccine Polymerase chain reaction |
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