血管紧张素转换酶抑制剂对缺血再灌注心肌细胞内钙超载的拮抗作用

目的：探讨血管紧张素转换酶抑制剂卡托普利和依那普利拉对心肌细胞内游离钙浓度的影响，并观察两药能否对抗缺血再灌注引起的细胞内钙超载作用。方法：Ｗｉｓｔａｒ大鼠心肌细胞培养７２小时后分为对照组、卡托普利组和依那普利拉组。药物于实验前３０分钟加入，终浓度为１０－５ｍｏｌ／Ｌ。Ｉｎｄｏ－１ＡＭ标记后，应用缺血再灌注损伤模型，用粘附式细胞仪连续观察缺血前、缺血及再灌注细胞内游离钙浓度（荧光比率）的变化。测试结果组间采用ｔ检验。结果：缺血前卡托普利组和依那普利拉组细胞内荧光比率均低于对照组（Ｐ＜０．０１～０．０５）；而缺血及再灌注阶段，对照组细胞内荧光比率持续升高，卡托普利组和依那普利拉组均保持相对平稳，显著低于对照组（Ｐ＜０．０１）。结论：血管紧张素转换酶抑制剂可以拮抗缺血再灌注心肌细胞内钙超载，对缺血再灌注心肌细胞具有明显的保护作用。

In Vitro Angiotensin Converting Enzyme Inhibitors Suppress [Ca 2 ]i Overload in Rat Cardiac Myocytes After Ischemia Reperfusion

Objective:The purpose of this study was to observe the effect of angiotensin converting enzyme inhibitors(ACEI) captopril (cap) and enalaprilat (ena) on [Ca 2 ]i overload of rat cardiac myocytes induced by ischemia reperfusion. Methods:Rat cardiac myocytes were cultured and divided into three groups (control,cap,ena)after 72 hours.Final concentration of ACEI (cap,ena) was 10 -5 mol/L.Rat cardiac myocytes were subjected to ischemia and reperfusion.Changes in [Ca 2 ]i were estimated using indo 1 AM as the [Ca 2 ]i indicator.Statistical analysis was performed by Student t test. Results:Treatment with captopril and enalaprilat led to decreasing of the [Ca 2 ]i concentration in cardiac myocytes ( p <0 01 0 05 vs.control group),which prevented [Ca 2 ]i overload induced by ischemia reperfusion ( p <0 01 vs.control group). Conclusion:Both captopril and enalaprilat could protect cardiac myocytes against ischemia reperfusion damage by suppressing [Ca 2 ]i overload in cardiac myocytes after ischemia reperfusion.