器官移植受者微量CMV DNA与IL-10基因单核苷酸多态性的相关性研究

目的研究白介素-10（IL-10）基因G1082A和C819T单核苷酸多态性（SNP）与移植术后外周血微量巨细胞病毒（CMV）DNA的关系。万法采集176例移植术后受者，429例次的外周血。通过巢式聚合酶链反应（nPcR）技术检测微量CMV DNA。通过序列特异性聚合酶链反应（SSP-PCR）技术检测IL-10基因G1082A与C819T的变异情况。分析IL-10各基因型在微量CMV DNA受者中的分布频率，并对阳性组与阴性组进行比较。结果研究人群中IL-10 G1082A和C819T基因型分布符合Hardy-Weinberg遗传平衡定律。CMV DNA阳性组IL-10 G1082A和C819T等位基因频率分别为G=3.75％、A=96.25％和C=32.50％、T=67.50％；CMV DNA阴性组分别为G=5.51％、A=94.49％和C=36.03％、T=63.97％。CMV DNA阳性组两SNP基因型及等位基因分布与其在CMV DNA阴性组中的分布差异无显著性（P〉0.05）。结论目前尚不能认为IL-10基因G1082A与C819T单核苷酸多态性与移植受者微量CMV DNA阳性有相关性。

Study on the correlation between the IL-10 gene's single nucleotide polymorphism (SNP) and the trace cytomegalovirus (CMV) DNA of recipients

Objective To investigate the relationship between the IL-10 G1082A and C819T 's SNP and the trace CMV DNA of recipients.Methods The trace CMV DNA in 429 samples of 176 patients was detected by nested polymerase chain reaction(nPCR).The IL-10 G1082A and C819T's SNP were determined using sequence specific primers polymerase chain reaction(SSP-PCR).Results In the 40 CMV DNA-positive samples,the G1082A allele frequencies were 3.75% for G and 96.25% for A,with the genotype frequencies 0% for G/G,92.5%(n=37)for A/A,and 7.5%(n=3)for G/A;In the 136 CMV DNA-negative samples,the G1082A allele frequencies were 5.51% for G and 94.49% for A,with the genotype frequencies 0% for G/G,88.97%(n=121)for A/A,and 11.03%(n=15)for G/A.In the CMV DNA-positive group,the C819T allele frequencies were 32.5% for C and 67.5% for T,with the genotype frequencies 5%(n=2)for C/C,40%(n=16)for T/T,and 55%(n=22)for C/T;In the CMV DNA-negative group,the IL-10 C819T allele frequencies were 36.03% for C and 63.97% for T,with the genotype frequencies 11.76%(n=16)for C/C,39.71%(n=54)for T/T,and 48.53%(n=66)for C/T.The two SNPs distribution of the frequencies were all statistically similar between the CMV DNA-positive/negative groups(P>0.05).Conclusions The IL-10 gene G1082A and C819T's SNP are not associated with the CMV DNA-positive recipients.